Surface-adsorbed silver half-shells as a platform for surface-enhanced immunoassays; optimization through morphological control
- 13 January 2009
- journal article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 134 (4), 776-783
- https://doi.org/10.1039/b808784a
Abstract
We have investigated the effectiveness of surface-adsorbed silver half-shells for inducing the surface-enhanced fluorescence phenomenon. Our simple structure consists of a dense monolayer of monodispersive latex sphere covered by thermally evaporated silver, some tens of nm thick. In order to increase its effectiveness as a platform for immunoassay, we explored three physical parameters; the diameter of the latex sphere, the deposition thickness and the adsorption density of the latex sphere. The maximum enhancement of 25.5 was achieved using the sphere with 56 nm diameter with 10 nm silver deposited. As for the adsorption density, the maximum fluorescence signal was achieved with a relatively sparse adsorption density, 12.5 per µm2vs. 105.9 per µm2, corresponding to the full coverage. With the adsorption density also optimized, the enhancement was further increased by a factor of three.Keywords
This publication has 28 references indexed in Scilit:
- Characterization of cap-shaped silver particles for surface-enhanced fluorescence effectsAnalytical Biochemistry, 2007
- Surface-Enhanced Raman Scattering from Individual Au Nanoparticles and Nanoparticle Dimer SubstratesNano Letters, 2005
- Advances in Surface-Enhanced FluorescenceJournal of Fluorescence, 2004
- Self-assembly of polystyrene nano particles into patterns of random-close-packed monolayers via chemically induced adsorptionPhysical Chemistry Chemical Physics, 2002
- Cap-shaped gold nanoparticles for an optical biosensorSensors and Actuators B: Chemical, 2000
- Surface-adsorbed polystyrene spheres as a template for nanosized metal particle formation: Optical properties of nanosized Au particleJournal of Vacuum Science & Technology B: Microelectronics and Nanometer Structures, 1999
- Enhancement of Molecular Fluorescence near the Surface of Colloidal Metal FilmsAnalytical Chemistry, 1998
- Probing Single Molecules and Single Nanoparticles by Surface-Enhanced Raman ScatteringScience, 1997
- Fluorescence enhancement from Langmuir-Blodgett monolayers on silver island filmsLangmuir, 1988
- Energy transfer in surface enhanced luminescenceThe Journal of Chemical Physics, 1983