An orthopoxvirus-based vaccine reduces virus excretion after MERS-CoV infection in dromedary camels
Top Cited Papers
- 1 January 2016
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 351 (6268), 77-81
- https://doi.org/10.1126/science.aad1283
Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) infections have led to an ongoing outbreak in humans, which was fueled by multiple zoonotic MERS-CoV introductions from dromedary camels. In addition to the implementation of hygiene measures to limit further camel-to-human and human-to-human transmissions, vaccine-mediated reduction of MERS-CoV spread from the animal reservoir may be envisaged. Here we show that a modified vaccinia virus Ankara (MVA) vaccine expressing the MERS-CoV spike protein confers mucosal immunity in dromedary camels. Compared with results for control animals, we observed a significant reduction of excreted infectious virus and viral RNA transcripts in vaccinated animals upon MERS-CoV challenge. Protection correlated with the presence of serum neutralizing antibodies to MERS-CoV. Induction of MVA-specific antibodies that cross-neutralize camelpox virus would also provide protection against camelpox.Keywords
Funding Information
- Nederlandse Organisatie voor Wetenschappelijk Onderzoek (91213066)
- Niedersachsen-Research Network on Neuroinfectiology (N-RENNT)
- Ministry of Science and Culture of Lower Saxony
- Innovative Medicines Initiative (IMI) (115760)
This publication has 35 references indexed in Scilit:
- Middle East Respiratory Syndrome Coronavirus Spike Protein Delivered by Modified Vaccinia Virus Ankara Efficiently Induces Virus-Neutralizing AntibodiesJournal of Virology, 2013
- Identification of a Receptor-Binding Domain in the S Protein of the Novel Human Coronavirus Middle East Respiratory Syndrome Coronavirus as an Essential Target for Vaccine DevelopmentJournal of Virology, 2013
- The Receptor Binding Domain of the New Middle East Respiratory Syndrome Coronavirus Maps to a 231-Residue Region in the Spike Protein That Efficiently Elicits Neutralizing AntibodiesJournal of Virology, 2013
- Hospital Outbreak of Middle East Respiratory Syndrome CoronavirusThe New England Journal of Medicine, 2013
- Dipeptidyl peptidase 4 is a functional receptor for the emerging human coronavirus-EMCNature, 2013
- Genomic Characterization of a Newly Discovered Coronavirus Associated with Acute Respiratory Distress Syndrome in HumansmBio, 2012
- Isolation of a Novel Coronavirus from a Man with Pneumonia in Saudi ArabiaThe New England Journal of Medicine, 2012
- Camelpox virusAntiviral Research, 2011
- Characterization of camelpoxvirus isolates from Africa and AsiaVeterinary Microbiology, 1995
- Development of a live cell culture camelpox vaccineVaccine, 1992