Isolation of a Novel Coronavirus from a Man with Pneumonia in Saudi Arabia
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- 8 November 2012
- journal article
- research article
- Published by Massachusetts Medical Society in The New England Journal of Medicine
- Vol. 367 (19), 1814-1820
- https://doi.org/10.1056/nejmoa1211721
Abstract
A previously unknown coronavirus was isolated from the sputum of a 60-year-old man who presented with acute pneumonia and subsequent renal failure with a fatal outcome in Saudi Arabia. The virus (called HCoV-EMC) replicated readily in cell culture, producing cytopathic effects of rounding, detachment, and syncytium formation. The virus represents a novel betacoronavirus species. The closest known relatives are bat coronaviruses HKU4 and HKU5. Here, the clinical data, virus isolation, and molecular identification are presented. The clinical picture was remarkably similar to that of the severe acute respiratory syndrome (SARS) outbreak in 2003 and reminds us that animal coronaviruses can cause severe disease in humans.Keywords
This publication has 21 references indexed in Scilit:
- A Family-Wide RT-PCR Assay for Detection of Paramyxoviruses and Application to a Large-Scale Surveillance StudyPLOS ONE, 2012
- Sensitive and Broadly Reactive Reverse Transcription-PCR Assays To Detect Novel ParamyxovirusesJournal of Clinical Microbiology, 2008
- A Pancoronavirus RT-PCR Assay for Detection of All Known CoronavirusesMethods in molecular biology (Clifton, N.J.), 2008
- Comparative Analysis of Twelve Genomes of Three Novel Group 2c and Group 2d Coronaviruses Reveals Unique Group and Subgroup FeaturesJournal of Virology, 2007
- Quantitative Real-Time PCR Assays for Detection of Human Adenoviruses and Identification of Serotypes 40 and 41Applied and Environmental Microbiology, 2005
- Characterization of a Novel Coronavirus Associated with Severe Acute Respiratory SyndromeScience, 2003
- Identification of a Novel Coronavirus in Patients with Severe Acute Respiratory SyndromeThe New England Journal of Medicine, 2003
- Coronavirus as a possible cause of severe acute respiratory syndromeThe Lancet, 2003
- Rapid Detection of Enterovirus RNA in Cerebrospinal Fluid Specimens with a Novel Single-Tube Real-Time Reverse Transcription-PCR AssayJournal of Clinical Microbiology, 2001
- Fingerprinting genomes using PCR with arbitrary primersNucleic Acids Research, 1990