Distinct activities of CHD1 and ACF in ATP-dependent chromatin assembly
- 9 January 2005
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Structural & Molecular Biology
- Vol. 12 (2), 160-166
- https://doi.org/10.1038/nsmb884
Abstract
CHD1 is a chromodomain-containing protein in the SNF2-like family of ATPases. Here we show that CHD1 exists predominantly as a monomer and functions as an ATP-utilizing chromatin assembly factor. This reaction involves purified CHD1, NAP1 chaperone, core histones and relaxed DNA. CHD1 catalyzes the ATP-dependent transfer of histones from the NAP1 chaperone to the DNA by a processive mechanism that yields regularly spaced nucleosomes. The comparative analysis of CHD1 and ACF revealed that CHD1 assembles chromatin with a shorter nucleosome repeat length than ACF. In addition, ACF, but not CHD1, can assemble chromatin containing histone H1, which is involved in the formation of higher-order chromatin structure and transcriptional repression. These results suggest a role for CHD1 in the assembly of active chromatin and a function of ACF in the assembly of repressive chromatin.Keywords
This publication has 50 references indexed in Scilit:
- Histone chaperones, a supporting role in the limelightBiochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 2003
- Chromatin assembly by DNA-translocating motorsNature Reviews Molecular Cell Biology, 2003
- Histone chaperones and nucleosome assemblyCurrent Opinion in Structural Biology, 2003
- HIRA Is Critical for a Nucleosome Assembly Pathway Independent of DNA SynthesisMolecular Cell, 2002
- Chromatin assemblyJBIC Journal of Biological Inorganic Chemistry, 2002
- The RCAF complex mediates chromatin assembly during DNA replication and repairNature, 1999
- ACF consists of two subunits, Acf1 and ISWI, that function cooperatively in the ATP-dependent catalysis of chromatin assemblyGenes & Development, 1999
- ACF, an ISWI-Containing and ATP-Utilizing Chromatin Assembly and Remodeling FactorCell, 1997
- Nucleosome Assembly by a Complex of CAF-1 and Acetylated Histones H3/H4Cell, 1996
- Drosophila NAP-1 Is a Core Histone Chaperone That Functions in ATP-Facilitated Assembly of Regularly Spaced Nucleosomal ArraysMolecular and Cellular Biology, 1996