Influenza Virus–induced Dendritic Cell Maturation Is Associated with the Induction of Strong T Cell Immunity to a Coadministered, Normally Nonimmunogenic Protein
Open Access
- 7 July 2003
- journal article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 198 (1), 133-144
- https://doi.org/10.1084/jem.20030266
Abstract
We evaluated the proposal that during microbial infection, dendritic cells (DCs) undergo maturation and present a mixture of peptides derived from the microbe as well as harmless environmental antigens. Mice were exposed to an aerosol of endotoxin free ovalbumin (OVA) in the absence or presence of influenza virus. In its absence, OVA failed to induce B and T cell responses and even tolerized, but with influenza, OVA-specific antibodies and CD8+ cytolytic T lymphocytes developed. With or without infection, OVA was presented selectively in the draining mediastinal lymph nodes, as assessed by the comparable proliferation of infused, CD8+ and CD4+, TCR transgenic T cells. In the absence of influenza, these OVA-specific T cells produced little IL-2, IL-4, IL-10, and IFN-γ, but with infection, both CD4+ and CD8+ T cells made high levels of IL-2 and IFN-γ. The OVA plus influenza-treated mice also showed accelerated recovery to a challenge with recombinant vaccinia OVA virus. CD11c+ DCs from the mediastinal lymph nodes of infected mice selectively stimulated both OVA- and influenza-specific T cells and underwent maturation, with higher levels of MHC class II, CD80, and CD86 molecules. The relatively slow (2–3 d) kinetics of maturation correlated closely to the time at which OVA inhalation elicited specific antibodies. Therefore respiratory infection can induce DC maturation and simultaneously B and T cell immunity to an innocuous antigen inhaled concurrently.Keywords
This publication has 42 references indexed in Scilit:
- Type I Interferon Induction Pathway, but Not Released Interferon, Participates in the Maturation of Dendritic Cells Induced by Negative?Strand RNA VirusesThe Journal of Infectious Diseases, 2003
- Efficient Targeting of Protein Antigen to the Dendritic Cell Receptor DEC-205 in the Steady State Leads to Antigen Presentation on Major Histocompatibility Complex Class I Products and Peripheral CD8+ T Cell ToleranceThe Journal of Experimental Medicine, 2002
- Lipopolysaccharide-enhanced, Toll-like Receptor 4–dependent T Helper Cell Type 2 Responses to Inhaled AntigenThe Journal of Experimental Medicine, 2002
- The CD8+ Dendritic Cell Subset Selectively Endocytoses Dying Cells in Culture and In VivoThe Journal of Experimental Medicine, 2002
- Dendritic Cells Induce Peripheral T Cell Unresponsiveness under Steady State Conditions in VivoThe Journal of Experimental Medicine, 2001
- Myeloid Dendritic Cells Stimulate Both Th1 and Th2 Immune Responses Depending on the Nature of the AntigenJournal of Interferon & Cytokine Research, 2001
- Immune Response Induced by Airway Sensitization after Influenza A Virus Infection Depends on Timing of Antigen Exposure in MiceJournal of Virology, 2001
- Intraepithelial airway dendritic cells: a distinct subset of pulmonary dendritic cells obtained by microdissection.The Journal of Experimental Medicine, 1992
- Dendritic cells are the principal cells in mouse spleen bearing immunogenic fragments of foreign proteins.The Journal of Experimental Medicine, 1990
- A Contiguous Network of Dendritic Antigen-Presenting Cells within the Respiratory EpitheliumInternational Archives of Allergy and Immunology, 1990