Nanosized Zinc Oxide Induces Toxicity in Human Lung Cells
Open Access
- 7 August 2013
- journal article
- research article
- Published by Hindawi Limited in ISRN Toxicology
- Vol. 2013, 1-8
- https://doi.org/10.1155/2013/316075
Abstract
Zinc oxide nanoparticles (ZnO-NPs) are increasingly used in sunscreens, biosensors, food additives, pigments, rubber manufacture, and electronic materials. With the wide application of ZnO-NPs, concern has been raised about its unintentional health and environmental impacts. This study investigates the toxic effects of ZnO-NPs in human lung cells. In order to assess toxicity, human lung epithelial cells (L-132) were exposed to dispersion of 50 nm ZnO-NPs at concentrations of 5, 25, 50, and 100 μg/mL for 24 h. The toxicity was evaluated by observing changes in cell morphology, cell viability, oxidative stress parameters, DNA damage analysis, and gene expression. Exposure to 50 nm ZnO-NPs at concentrations between 5 and 100 μg/mL decreased cell viability in a concentration-dependent manner. Morphological examination revealed cell shrinkage, nuclear condensation, and formation of apoptotic bodies. The oxidative stress parameters revealed significant depletion of GSH level and increase in ROS levels suggesting generation of oxidative stress. ZnO-NPs exposure caused DNA fragmentation demonstrating apoptotic type of cell death. ZnO-NPs increased the expression of metallothionein gene, which is considered as a biomarker in metal-induced toxicity. To summarize, ZnO-NPs cause toxicity in human lung cells possibly through oxidative stress-induced apoptosis.Keywords
This publication has 45 references indexed in Scilit:
- ZnO nanorod-induced apoptosis in human alveolar adenocarcinoma cells via p53, survivin and bax/bcl-2 pathways: role of oxidative stressNanomedicine: Nanotechnology, Biology and Medicine, 2011
- Comparative toxicity of 24 manufactured nanoparticles in human alveolar epithelial and macrophage cell linesParticle and Fibre Toxicology, 2009
- Exposure to titanium dioxide and other metallic oxide nanoparticles induces cytotoxicity on human neural cells and fibroblastsInternational Journal of Nanomedicine, 2008
- Pulmonary applications and toxicity of engineered nanoparticlesAmerican Journal of Physiology-Lung Cellular and Molecular Physiology, 2008
- Preferential killing of cancer cells and activated human T cells using ZnO nanoparticlesNanotechnology, 2008
- Ambient particle inhalation and the cardiovascular system: potential mechanisms.Environmental Health Perspectives, 2001
- Is Nanotechnology Dangerous?Science, 2000
- Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate readerFree Radical Biology & Medicine, 1999
- Metal Fume FeverJournal of Occupational and Environmental Medicine, 1997
- Possible role for metallothionein in protection against radiation-induced oxidative stress. Kinetics and mechanism of its reaction with superoxide and hydroxyl radicalsBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1985