Comparative toxicity of 24 manufactured nanoparticles in human alveolar epithelial and macrophage cell lines
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Open Access
- 1 January 2009
- journal article
- research article
- Published by Springer Science and Business Media LLC in Particle and Fibre Toxicology
- Vol. 6 (1), 14
- https://doi.org/10.1186/1743-8977-6-14
Abstract
A critical issue with nanomaterials is the clear understanding of their potential toxicity. We evaluated the toxic effect of 24 nanoparticles of similar equivalent spherical diameter and various elemental compositions on 2 human pulmonary cell lines: A549 and THP-1. A secondary aim was to elaborate a generic experimental set-up that would allow the rapid screening of cytotoxic effect of nanoparticles. We therefore compared 2 cytotoxicity assays (MTT and Neutral Red) and analyzed 2 time points (3 and 24 hours) for each cell type and nanoparticle. When possible, TC50 (Toxic Concentration 50 i.e. nanoparticle concentration inducing 50% cell mortality) was calculated.Keywords
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