Global DNA Demethylation During Mouse Erythropoiesis in Vivo
- 11 November 2011
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 334 (6057), 799-802
- https://doi.org/10.1126/science.1207306
Abstract
In the mammalian genome, 5′-CpG-3′ dinucleotides are frequently methylated, correlating with transcriptional silencing. Genome-wide demethylation is thought to occur only twice during development, in primordial germ cells and in the pre-implantation embryo. These demethylation events are followed by de novo methylation, setting up a pattern inherited throughout development and modified only at tissue-specific loci. We studied DNA methylation in differentiating mouse erythroblasts in vivo by using genomic-scale reduced representation bisulfite sequencing (RRBS). Demethylation at the erythroid-specific β-globin locus was coincident with global DNA demethylation at most genomic elements. Global demethylation was continuous throughout differentiation and required rapid DNA replication. Hence, DNA demethylation can occur globally during somatic cell differentiation, providing an experimental model for its study in development and disease.This publication has 34 references indexed in Scilit:
- A Key Commitment Step in Erythropoiesis Is Synchronized with the Cell Cycle Clock through Mutual Inhibition between PU.1 and S-Phase ProgressionPLoS Biology, 2010
- BISMA - Fast and accurate bisulfite sequencing data analysis of individual clones from unique and repetitive sequencesBMC Bioinformatics, 2010
- Genome-wide erasure of DNA methylation in mouse primordial germ cells is affected by AID deficiencyNature, 2010
- Reprogramming towards pluripotency requires AID-dependent DNA demethylationNature, 2009
- Rethinking how DNA methylation patterns are maintainedNature Reviews Genetics, 2009
- Histone modifications at human enhancers reflect global cell-type-specific gene expressionNature, 2009
- DNA Demethylation in Zebrafish Involves the Coupling of a Deaminase, a Glycosylase, and Gadd45Cell, 2008
- Genome-scale DNA methylation maps of pluripotent and differentiated cellsNature, 2008
- Enucleation of primitive erythroid cells generates a transient population of “pyrenocytes” in the mammalian fetusPublished by American Society of Hematology ,2008
- Negative Autoregulation by FAS Mediates Robust Fetal ErythropoiesisPLoS Biology, 2007