Reprogramming towards pluripotency requires AID-dependent DNA demethylation
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- 21 December 2009
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 463 (7284), 1042-1047
- https://doi.org/10.1038/nature08752
Abstract
Reprogramming of somatic cell nuclei to yield induced pluripotent stem (iPS) cells makes possible derivation of patient-specific stem cells for regenerative medicine. However, iPS cell generation is asynchronous and slow (2–3 weeks), the frequency is low (OCT4 (also known as POU5F1) and NANOG gene expression. AID protein bound silent methylated OCT4 and NANOG promoters in fibroblasts, but not active demethylated promoters in ES cells. These data provide new evidence that mammalian AID is required for active DNA demethylation and initiation of nuclear reprogramming towards pluripotency in human somatic cells.Keywords
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