Role for cyclin A in the dependence of mitosis on completion of DMA replication
- 1 November 1991
- journal article
- letter
- Published by Springer Science and Business Media LLC in Nature
- Vol. 354 (6351), 314-317
- https://doi.org/10.1038/354314a0
Abstract
THE cyclins were first identified by their cell-cycle-dependent synthesis and destruction1–3 and have a key role in the control of mitosis in Xenopusembryonic cell cycles4–6. All higher eukaryotes have at least two types of cyclins, the A- and B-type, which can be distinguished by sequence motifs and the timing of their destruction in the cell cycle2,7–10. The degradation of both cyclins is required for exit from mitosis11, but the activation and destruction of cyclin A occur earlier in the cell cycle than with the B-type cyclins9–11. This suggests that cyclin A has a distinct role in cell-cycle progression. We have used an antisense oligodeoxy-nucleotide directed against cyclin A to investigate this role. Ablation of cyclin A messenger RNA in cytostatic factor/metaphase-arrested extracts of Xenopus eggs, followed by in vitro progression into interphase, resulted in the premature appearance of cyclin B/cdc2-associated H1 kinase activity and premature entry into mitosis. Although cyclin A-ablated extracts could initiate DNA synthesis during interphase, S phase was not completed before entry into mitosis. The effects of cyclin A ablation were reversed by the addition of cyclin A mRNA or cyclin A protein to the extracts.Keywords
This publication has 21 references indexed in Scilit:
- Activation of p34cdc2 kinase by cyclin A.The Journal of cell biology, 1991
- Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from cyclin BNature, 1990
- Completion of DNA replication is monitored by a feedback system that controls the initiation of mitosis in vitro: Studies in XenopusCell, 1990
- The role of cyclin synthesis and degradation in the control of maturation promoting factor activityNature, 1989
- Cyclin synthesis drives the early embryonic cell cycleNature, 1989
- Expression and function of Drosophila cyclin a during embryonic cell cycle progressionCell, 1989
- Translation of cyclin mRNA is necessary for extracts of activated Xenopus eggs to enter mitosisCell, 1989
- Cyclin synthesis, modification and destruction during meiotic maturation of the starfish oocyteDevelopmental Biology, 1987
- The clam embryo protein cyclin A induces entry into M phase and the resumption of meiosis in Xenopus oocytesCell, 1986
- Cyclin: A protein specified by maternal mRNA in sea urchin eggs that is destroyed at each cleavage divisionCell, 1983