Gymnema montanum H. Protects Against Alloxan-induced Oxidative Stress and Apoptosis in Pancreatic β-cells
Open Access
- 1 January 2009
- journal article
- research article
- Published by S. Karger AG in Cellular Physiology and Biochemistry
- Vol. 24 (5-6), 429-440
- https://doi.org/10.1159/000257480
Abstract
The present study evaluated the molecular mechanism of antidiabetic property of G. montanum leaf extract (GLEt) against alloxan-induced apoptotic cell death in rat insulinoma cells (RINm5F). The pre-treatment of GLEt (5 μg and 10 μg/ml) resulted in significant decrease in intracellular Ca2+ concentration, nitric oxide (NO) production along with increase in mitochondrial membrane potential in alloxan (7mM/ml) treated cells. Further GLEt reduced apoptosis by inhibiting the release of cytochrome c and subsequent cleavage of PARP and caspase-3. The immunochemical staining of 8-hydroxydeoxyguanosine (8-OHdG) also evidenced the suppression of oxidative stress by GLEt. The cell cycle analysis, annexin-V labelling assay and TUNEL assay showed the suppression of apoptosis by the treatment of GLEt. Moreover, GLEt significantly increased the cellular antioxidant levels and decreased the lipid peroxides in alloxan-treated RINm5F cells. Taken together, these findings suggest that G. montanum protects pancreatic β-cells against reactive oxygen species (ROS) by counteracting with mitochondrial membrane permeability and inhibition of the apoptotic pathway.Keywords
This publication has 13 references indexed in Scilit:
- Antihyperlipidaemic Effect of Gymnema montanum: A Study on Lipid Profile and Fatty Acid Composition in Experimental DiabetesBasic & Clinical Pharmacology & Toxicology, 2008
- Curcumin prevents streptozotocin-induced islet damage by scavenging free radicals: A prophylactic and protective roleEuropean Journal of Pharmacology, 2007
- Effect of Gymnema montanum leaves on red blood cell resistance to oxidative stress in experimental diabetesCell Biology and Toxicology, 2007
- Protective effects of an herbal formulation of Radix Astragali, Radix Codonopsis and Cortex Lycii on streptozotocin‐induced apoptosis in pancreatic β‐cells: an implication for its treatment of diabetes mellitusPhytotherapy Research, 2007
- Metabolic Disorders in Diabetes Mellitus: Impact of Mitochondrial Function and Oxidative Stress on Diabetes and Its ComplicationsAntioxidants and Redox Signaling, 2007
- Glycemic Control of Apoptosis in the Pancreatic Beta Cell: Danger of Extremes?Antioxidants and Redox Signaling, 2007
- Ethanol extract of Gymnema montanum leaves reduces glycoprotein components in experimental diabetesNutrition Research, 2007
- Iptakalim protects PC12 cell against H2O2-induced oxidative injury via opening mitochondrial ATP-sensitive potassium channelBiochemical and Biophysical Research Communications, 2006
- Relative importance of cellular uptake and reactive oxygen species for the toxicity of alloxan and dialuric acid to insulin-producing cellsFree Radical Biology & Medicine, 2006
- High glucose‐induced oxidative stress causes apoptosis in proximal tubular epithelial cells and is mediated by multiple caspasesThe FASEB Journal, 2003