Effect of Gymnema montanum leaves on red blood cell resistance to oxidative stress in experimental diabetes
- 19 September 2007
- journal article
- Published by Springer Science and Business Media LLC in Cell Biology and Toxicology
- Vol. 24 (3), 233-241
- https://doi.org/10.1007/s10565-007-9032-z
Abstract
The aim of the present study was to evaluate the protective effect of Gymnema montanum on red blood cell (RBC) membrane in diabetic rats during lipid peroxidation. Ethanol extract of G. montanum leaves (GLEt) was administered orally to alloxan-induced diabetic rats for 3 weeks, and the effects on blood glucose, insulin, lipid peroxidation markers, thiobarbituric acid reactive substances, hydroperoxides in plasma and antioxidant enzymes including superoxide dismutase, catalase and glutathione peroxidase activities in erythrocytes were studied. Administration of GLEt to diabetic animals at doses of 50, 100, and 200 mg/kg body weight lowered elevated blood glucose levels by 24, 35, and 66%, respectively, relative to untreated diabetic rats. In comparison, treatment with the known antidiabetic drug, glibenclamide (600 μg/kg body weight) decreased blood glucose concentrations by 51%. Plasma insulin concentrations were increased in the diabetic rat by 73% with GLEt (200 mg/kg body weight) and 45% with glibenclamide (600 μg/kg body weight). Although a significant decrease in the lipid peroxidation markers was observed in plasma on treatment with GLEt and glibenclamide, the RBC antioxidant levels were increased significantly in diabetic rats. Furthermore, erythrocytes from the GLEt-treated animals were found to be more resistant to H2O2-induced peroxidation than that of untreated diabetic animals. The chemical characterization of the polyphenolics of the extract showed the presence of gallic acid (5.29% w/w), resveratrol (2.2% w/w), and quercetin (16.6% w/w). The results of this study suggest that G. montanum may be useful for the control, management, and prevention of oxidative stress associated with diabetes.Keywords
This publication has 22 references indexed in Scilit:
- Modulatory effects of gymnema montanum leaf extract on alloxan-induced oxidative stress in wistar ratsNutrition, 2004
- Effect ofGymnema montanumon Blood Glucose, Plasma Insulin, and Carbohydrate Metabolic Enzymes in Alloxan-Induced Diabetic RatsJournal of Medicinal Food, 2003
- β-Cell Expansion for Therapeutic Compensation of Insulin Resistance in Type 2 DiabetesExperimental Diabesity Research, 2003
- Medicinal plants of India with anti-diabetic potentialJournal of Ethnopharmacology, 2002
- Lipid peroxidation and antioxidant status in experimental diabetesClinica Chimica Acta; International Journal of Clinical Chemistry, 1999
- Role of oxidative stress in diabetic complications: a new perspective on an old paradigm.Diabetes, 1999
- Hypoglycaemic effect of Artemisia herba alba. II. Effect of a valuable extract on some blood parameters in diabetic animalsJournal of Ethnopharmacology, 1994
- Role of Oxidative Stress in Development of Complications in DiabetesDiabetes, 1991
- Erythrocyte malondialdehyde release in vitro: A functional measure of vitamin E statusClinica Chimica Acta; International Journal of Clinical Chemistry, 1985
- CARBOHYDRATE UTILIZATIONJournal of Biological Chemistry, 1925