Secretogranin II Is Synthesized and Secreted in Astrocyte Cultures
- 1 June 1993
- journal article
- Published by Wiley in Journal of Neurochemistry
- Vol. 60 (6), 2312-2314
- https://doi.org/10.1111/j.1471-4159.1993.tb03520.x
Abstract
Astrocyte cultures from rat brain were analyzed for their ability to synthesize and secrete secretogranin II (chromogranin C). Northern blot analysis of polyA-selected RNA established the presence of secretogranin II mRNA in these cells. By radioimmunoassay, 11.6 fmol/10(6) astrocytes of secretogranin II was found in these cells. About twice the amount was released into the medium within 3 days. Secretogranin II within the astrocytes was practically unprocessed, as shown by HPLC. These results establish for the first time that astrocytes in vitro synthesize and secrete a protein of the acidic chromogranin family.Keywords
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