Catalase T and Cu, Zn‐superoxide dismutase in the acetic acid‐induced programmed cell death inSaccharomyces cerevisiae
- 17 December 2007
- journal article
- Published by Wiley in FEBS Letters
- Vol. 582 (2), 210-214
- https://doi.org/10.1016/j.febslet.2007.12.007
Abstract
To investigate the role of catalase and superoxide dismutase (SOD) in the acetic acid (AA) induced yeast programmed cell death (AA-PCD), we compared Saccharomyces cerevisiae cells (C-Y) and cells individually over-expressing catalase T (CTT1-Y) and Cu,Zn-SOD (SOD1-Y) with respect to cell survival, hydrogen peroxide (H2O2) levels and enzyme activity as measured up to 200 min after AA treatment. AA-PCD does not occur in CTT1-Y, where H2O2 levels were lower than in C-Y and the over-expressed catalase activity decreased with time. In SOD1-Y, AA-PCD was exacerbated; high H2O2 levels were found, SOD activity increased early, remaining constant en route to AA-PCD, but catalase activity was strongly reduced.Keywords
This publication has 25 references indexed in Scilit:
- Protective role of mitochondrial superoxide dismutase against high osmolarity, heat and metalloid stress inSaccharomyces cerevisiaeFolia Microbiologica, 2007
- Yeast apoptosis—From genes to pathwaysSeminars in Cancer Biology, 2006
- YCA1 participates in the acetic acid induced yeast programmed cell death also in a manner unrelated to its caspase‐like activityFEBS Letters, 2006
- Redox Control of Apoptosis: An UpdateAntioxidants and Redox Signaling, 2006
- Ysp2 mediates death of yeast induced by amiodarone or intracellular acidificationBiochimica et Biophysica Acta (BBA) - Bioenergetics, 2006
- Diethyldithiocarbamate inhibits in vivo Cu,Zn-superoxide dismutase and perturbs free radical processes in the yeast Saccharomyces cerevisiae cellsBiochemical and Biophysical Research Communications, 2005
- Acid stress adaptation protects Saccharomyces cerevisiae from acetic acid-induced programmed cell deathGene, 2005
- Role of mitochondria in the pheromone- and amiodarone-induced programmed death of yeastThe Journal of cell biology, 2005
- Apoptosis in yeastCurrent Opinion in Microbiology, 2004
- Production of Reactive Oxygen Species, Alteration of Cytosolic Ascorbate Peroxidase, and Impairment of Mitochondrial Metabolism Are Early Events in Heat Shock-Induced Programmed Cell Death in Tobacco Bright-Yellow 2 CellsPlant Physiology, 2004