Dissociation kinetics of 19 base paired oligonucleotide-DNA duplexes containing different single mismatched base pairs

Abstract

The dissociation kinetics of 19 base paired oligonucleotide-DNA duplexcontaining a various single mismatched base pair are studied on dried agarose gels. The kinetics of the dissociation are first order under our experimental conditions. The incorporation of a single mismatched base pair destabilizes the DNA duplexes to some extent, the amount depending on the nature of the mismatched base pair. G-T and G-A mismatches slightly destabilize a duplex, while A-A, T-T, C-T and C-A mismatches significantly destabilize it. The activation energy for the overall dissociation processes for these oligonucleotide-DNA duplexes containing 19 base pairs is 52 ± 2 Kcalmol⁻¹ as determined from the slope of Arrhenius plot.