Oligonucleotide directed mutagenesis of the human β-globin gene: a general method for producing specific point mutations in cloned DNA
Open Access
- 11 August 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 9 (15), 3647-3656
- https://doi.org/10.1093/nar/9.15.3647
Abstract
A nonadecanucleotide has been used both as a site specific mutagen to introduce an T→-A transversion mutation in the human β-globin gene cloned in pBR322 as well as a probe to screen transformed colonies for the desired mutant. The specificity of the oligonucleotide as a mutagen and as a hybridization probe provide a general method for producing site specific mutations in DNA cloned in plasmid vectors such as pBR322.Keywords
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