Differential regulation of cytochrome P450 3A1 and P450 3A2 in rat liver following dexamethasone treatment
- 1 October 1995
- journal article
- research article
- Published by Wiley in Journal of Biochemical Toxicology
- Vol. 10 (6), 299-307
- https://doi.org/10.1002/jbt.2570100604
Abstract
Induction of P450 3A1 and P450 3A2 was studied in adult rat liver following treatment with a single high dose of dexamethasone (DEX). The increase of both P450 3A1 and 3A2 occurred at the level of mRNA as well as protein. P450 3A isozymes thus induced were catalytically active. No constitutive expression of P450 3A1 mRNA or protein was observed in males or females. Constitutive expression of P450 3A2 mRNA and protein was observed in males but not in females. Additionally, in females, P450 3A2 was almost nondetectable compared to that in males, at any dose of DEX. A time course study following DEX treatment showed that P450 3A1 mRNA and protein were detectable in both sexes at 12 hours, increased until 48 hours, and then declined. The decline was more rapid in males. P450 3A2 mRNA and protein increased as early as 3 hours, increased further up to 48 hours, and slowly declined thereafter. A dose-response study indicated that P450 3A1 mRNA and protein progressively increased in both sexes from a dose of 30 mg/kg. In contrast, P450 3A2 mRNA and protein in males did not increase up to a dose of 30 mg/kg but increased at higher doses. Total P450 content and P450 3A catalytic activity were also found to increase with time and dose. © 1996 John Wiley & Sons, Inc.Keywords
This publication has 30 references indexed in Scilit:
- Lounging in a lysosome: the intracellular lifestyle of Coxiella burnetiiCellular Microbiology, 2007
- Constitutive Expression of Metallothionein Genes in Mouse BrainToxicology and Applied Pharmacology, 1995
- Differential Expression of the Metallothionein Gene in Liver and Brain of Mice and RatsToxicology and Applied Pharmacology, 1993
- The P450 Superfamily: Update on New Sequences, Gene Mapping, Accession Numbers, Early Trivial Names of Enzymes, and NomenclatureDNA and Cell Biology, 1993
- Differentiation requires continuous regulation.The Journal of cell biology, 1991
- Role of Cytochromes P45O in Drug Metabolism and HepatotoxicitySeminars in Liver Disease, 1990
- METALLOTHIONEINAnnual Review of Biochemistry, 1986
- Variety in the level of gene control in eukaryotic cellsNature, 1982
- Pregnenolone-16α-carbonitrile: A new type of inducer of drug-metabolizing enzymesArchives of Biochemistry and Biophysics, 1972
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970