DNA recombination during PCR
Open Access
- 1 January 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 18 (7), 1687-1691
- https://doi.org/10.1093/nar/18.7.1687
Abstract
PCR co-amplification of two distinct HIV1 tat gene sequences lead to the formation of recombinant DNA molecules. The frequency of such recombinants, up to 5.4% of all amplified molecules, could be decreased 2.7 fold by a 6 fold increase In Taq DNA polymerase elongatlon time. Crossover sites mapped essentially to three discrete regions suggesting specific Taq DNA polymerase pause or termination sites. PCR mediated recombination may be a problem when studying heterogeneous genetic material such as RNA viruses, multigene families, or repetitive sequences. This phenomenon can be exploited to create chimeric molecules from related sequences.Keywords
This publication has 9 references indexed in Scilit:
- Incomplete primer extension duringin vitroDNA amplification catalyzed byTaqpolymerase; exploitation for DNA sequencingNucleic Acids Research, 1989
- Temporal fluctuations in HIV quasispecies in vivo are not reflected by sequential HIV isolationsCell, 1989
- HIV-1 isolates are rapidly evolving quasispecies: evidence for viral mixtures and preferred nucleotide substitutions.1989
- Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA PolymeraseScience, 1988
- [21] Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reactionMethods in Enzymology, 1987
- Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell AnemiaScience, 1985
- Nucleotide sequence of the AIDS virus, LAVCell, 1985
- Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.Proceedings of the National Academy of Sciences of the United States of America, 1983
- Optimal computer folding of large RNA sequences using thermodynamics and auxiliary informationNucleic Acids Research, 1981