The neurogenic suppressor of hairless DNA-binding protein mediates the transcriptional activation of the enhancer of split complex genes triggered by Notch signaling.
- 1 November 1995
- journal article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 9 (21), 2598-2608
- https://doi.org/10.1101/gad.9.21.2598
Abstract
The Notch protein (N) acts as a transmembrane receptor for intercellular signals controlling cell fate choices in vertebrates and invertebrates. The signal of N activation may be transduced directly from the cell surface into the nucleus by an evolutionarily conserved transcription factor, Suppressor of Hairless [Su(H)], by its regulated nuclear import. Su(H) is shown here to play a direct role in the immediate response of the genome to N signaling in Drosophila. First, Su(H) mutant embryos derived from mutant germ-line clones exhibited a "neurogenic" phenotype of neural hypertrophy similar to the N phenotype. Second, the lack of N lateral signaling in these Su(H) mutant embryos was associated with a failure to express the m5 and m8 genes from the Enhancer of split Complex [E(spl)-C]. Finally, the Su(H) protein bound to the regulatory sequences of the E(spl)-C m5 and m8 genes, and these binding sites were required for the activation of the m5 and m8 promoters in the ventral neuroectoderm. The expression of the E(spl)-C m8 gene was found to be similarly regulated by Su(H) during wing imaginal disc development. Thus, the transcriptional activation of these E(spl)-C genes by Su(H) appears to be a direct and relatively general response to the activation of N. However, we also present evidence indicating that N signals in an Su(H)-independent manner during mesectoderm formation.Keywords
This publication has 29 references indexed in Scilit:
- Signalling downstream of activated mammalian NotchNature, 1995
- Masking of the CBF1/RBPJ κ Transcriptional Repression Domain by Epstein-Barr Virus EBNA2Science, 1995
- The torso receptor tyrosine kinase can activate raf in a ras-independent pathwayCell, 1995
- The suppressor of hairless protein participates in notch receptor signalingCell, 1994
- Mediation of Epstein-Barr Virus EBNA2 Transactivation by Recombination Signal-Binding Protein J κScience, 1994
- Roles of the Notch gene in Drosophila wing morphogenesisMechanisms of Development, 1994
- The Drosophila single-minded gene encodes a helix-loop-helix protein that acts as a master regulator of CNS midline developmentCell, 1991
- Specific EGF repeats of Notch mediate interactions with Delta and serrate: Implications for notch as a multifunctional receptorCell, 1991
- A protein binding to the Jk recombination sequence of immunoglobulin genes contains a sequence related to the integrase motifNature, 1989
- Vectors for Drosophila P-element-mediated transformation and tissue culture transfectionGene, 1988