Mediation of Epstein-Barr Virus EBNA2 Transactivation by Recombination Signal-Binding Protein J κ
- 1 July 1994
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 265 (5168), 92-95
- https://doi.org/10.1126/science.8016657
Abstract
The Epstein-Barr virus (EBV) transactivator protein, termed Epstein-Barr virus nuclear antigen 2 (EBNA2), plays a critical role in the regulation of latent viral transcription and in the immortalization of EBV-infected B cells. Unlike most transcription factors, EBNA2 does not bind directly to its cis-responsive DNA element but requires a cellular factor, termed C-promoter binding factor 1 (CBF1). Here, CBF1 was purified and was found to directly interact with EBNA2. CBF1 is identical to a protein thought to be involved in immunoglobulin gene rearrangement, RBPJ kappa. Contrary to previous reports, CBF1-RBPJ kappa did not bind to the recombination signal sequences but instead bound to sites in the EBV C-promoter and in the CD23 promoter.Keywords
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