A RAPID METHOD FOR DETERMINING BLOOD VOLUMES BY THE USE OF P32-LABELLED RED CELLS
Open Access
- 1 March 1950
- journal article
- research article
- Published by American Society for Clinical Investigation in JCI Insight
- Vol. 29 (3), 313-316
- https://doi.org/10.1172/jci102259
Abstract
6-8 ml. of a subject''s own blood is incubated for 30 min. with 100[um] curies P32. The cells are then washed, resuspended in sterile buffer and a measured aliquot injd. After 10-20 min. a blood sample is withdrawn and its radioactivity measured on a Geiger counter above a cup filled to a depth of over 7 mm. with the sample. This is compared with the measurement of an 18% glucose soln. dilution of the original suspension, giving the blood volume. The technic reduces errors due to plasma p32 variations, erythrocyte P32 loss, and sample beta-ray self-absorption, furnishing a method suitable for routine detns.This publication has 12 references indexed in Scilit:
- BLOOD VOLUME DETERMINATION IN THE HUMAN WITH RED CELLS CONTAINING RADIOACTIVE PHOSPHORUS (P32) AND WITH PURE HUMAN ALBUMIN 1JCI Insight, 1948
- THE DISTRIBUTION OF RED CELLS AND PLASMA IN LARGE AND MINUTE VESSELS OF THE NORMAL DOG, DETERMINED BY RADIOACTIVE ISOTOPES OF IRON AND IODINE 1JCI Insight, 1946
- CIRCULATING RED CELL VOLUME MEASURED SIMULTANEOUSLY BY THE RADIOACTIVE IRON AND DYE METHODS 1JCI Insight, 1946
- THE USE OF TWO RADIOACTIVE ISOTOPES OF IRON IN TRACER STUDIES OF ERYTHROCYTES 1JCI Insight, 1946
- THE MEASUREMENT OF THE CIRCULATING RED CELL VOLUME BY MEANS OF TWO RADIOACTIVE ISOTOPES OF IRON 1JCI Insight, 1946
- BLOOD VOLUME DETERMINATIONS WITH RADIOACTIVE PHOSPHORUSHeart, 1945
- SIMULTANEOUS MEASUREMENTS OF THE BLOOD VOLUME IN MAN AND DOG BY MEANS OF EVANS BLUE DYE, T1824, AND BY MEANS OF CARBON MONOXIDE. I. NORMAL SUBJECTS 1JCI Insight, 1944
- SIMULTANEOUS MEASUREMENTS OF THE BLOOD VOLUME IN MAN AND DOG BY MEANS OF EVANS BLUE DYE, T1824, AND BY MEANS OF CARBON MONOXIDE. II. UNDER ABNORMAL CONDITIONS, INCLUDING SECONDARY SHOCK 1JCI Insight, 1944
- Red Cell Volume Circulating and Total as Determined by Radio IronScience, 1941
- THE DETERMINATION OF THE CIRCULATING BLOOD VOLUME WITH CARBON MONOXIDEJCI Insight, 1928