Evaluation of the antioxidant effects of four main theaflavin derivatives through chemiluminescence and DNA damage analyses
Open Access
- 4 September 2011
- journal article
- Published by Zhejiang University Press in Journal of Zhejiang University-SCIENCE B
- Vol. 12 (9), 744-751
- https://doi.org/10.1631/jzus.B1100041
Abstract
Theaflavins (TFs) are the dimers of a couple of epimerized catechins, which are specially formed during black tea fermentation. To explore the differences among four main TF derivatives (theaflavin (TF1), theaflavin-3-gallate (TF2A), theaflavin-3′-gallate (TF2B), and theaflavin-3,3′-digallate (TF3)) in scavenging reactive oxygen species (ROS) in vitro, their properties of inhibiting superoxide, singlet oxygen, hydrogen peroxide, and the hydroxyl radical, and their effects on hydroxyl radical-induced DNA oxidative damage were systematically analyzed in the present study. The results show that, compared with (−)-epigallocatechin gallate (EGCG), TF derivatives were good antioxidants for scavenging ROS and preventing the hydroxyl radical-induced DNA damage in vitro. TF3 was the most positive in scavenging hydrogen peroxide and hydroxyl radical, and TF1 suppressed superoxide. Positive antioxidant capacities of TF2B on singlet oxygen, hydrogen peroxide, hydroxyl radical, and the hydroxyl radical-induced DNA damage in vitro were found. The differences between the antioxidant capacities of four main TF derivatives in relation to their chemical structures were also discussed. We suggest that these activity differences among TF derivatives would be beneficial to scavenge different ROS with therapeutic potential.Keywords
This publication has 32 references indexed in Scilit:
- ISOLATION AND PURIFICATION OF FOUR INDIVIDUAL THEAFLAVINS USING SEMI-PREPARATIVE HIGH PERFORMANCE LIQUID CHROMATOGRAPHYJournal of Liquid Chromatography & Related Technologies, 2010
- Synergistic effects of tea polyphenols and ascorbic acid on human lung adenocarcinoma SPC-A-1 cellsJournal of Zhejiang University-SCIENCE B, 2010
- Cancer prevention by tea: animal studies, molecular mechanisms and human relevanceNature Reviews Cancer, 2009
- Stability of Black Tea Polyphenol, Theaflavin, and Identification of Theanaphthoquinone as Its Major Radical Reaction ProductJournal of Agricultural and Food Chemistry, 2005
- Optimization of theaflavin biosynthesis from tea polyphenols using an immobilized enzyme system and response surface methodologyBiotechnology Letters, 2005
- Chemiluminescence assay for reactive oxygen species scavenging activities and inhibition on oxidative damage of DNA in Deinococcus radioduransLuminescence, 2004
- Green tea and cancer in humans: A review of the literatureNutrition and Cancer, 1998
- The chemistry of tea flavonoidsCritical Reviews in Food Science and Nutrition, 1997
- Antioxidant properties of phenolic compoundsTrends in Plant Science, 1997
- The antioxidant properties of theaflavins and their gallate esters — radical scavengers or metal chelators?FEBS Letters, 1996