Optimization of theaflavin biosynthesis from tea polyphenols using an immobilized enzyme system and response surface methodology
- 1 February 2005
- journal article
- research article
- Published by Springer Science and Business Media LLC in Biotechnology Letters
- Vol. 27 (4), 269-274
- https://doi.org/10.1007/s10529-004-8292-4
Abstract
Theaflavins were synthesized from tea polyphenols extracted from green tea using an immobilized polyphenol oxidase system. To optimize the production of theaflavins, response surface methodology was applied to determine the effects of five critical variables and their mutual interactions on theaflavin biosynthesis at five levels. A total of 52 individual experiments were performed and a statistical model predicted that the highest theaflavin concentration was 0.766 mg ml−1 at optimized conditions. Using these optimal parameters under experimental conditions in three independent replicates, the average value of the biosynthesized theaflavin concentration reached 0.75 ± 0.017 mg ml−1 and matched the value predicted by the modelKeywords
This publication has 6 references indexed in Scilit:
- The Theaflavin Monomers Inhibit the Cancer Cells Growth in VitroActa Biochimica et Biophysica Sinica, 2004
- Enzymatic synthesis of tea theaflavin derivatives and their anti-inflammatory and cytotoxic activitiesBioorganic & Medicinal Chemistry, 2003
- Comparative antimutagenic and anticlastogenic effects of green tea and black tea: a reviewMutation Research, 2002
- Theaflavate B, ISOTHEAFLAVIN-3′-O-GALLATE and NEOTHEAFLAVIN-3-O-GALLATE: three polyphenolic pigments from black teaPhytochemistry, 1998
- Effects of catechin concentration on the formation of black tea polyphenols during in vitro oxidationPhytochemistry, 1983
- Effects of physical and chemical conditions on the in vitro oxidation of tea leaf catechinsPhytochemistry, 1983