Expression of growth factor ligand and receptor genes in the preimplantation bovine embryo
- 1 February 1992
- journal article
- research article
- Published by Wiley in Molecular Reproduction and Development
- Vol. 31 (2), 87-95
- https://doi.org/10.1002/mrd.1080310202
Abstract
The sensitive technique of mRNA phenotyping with the reverse transcription‐polymerase chain reaction was employed to determine the patterns of gene expression for several growth factor ligand and receptor genes during bovine preimplantation development. Several thousand bovine embryos encompassing a developmental series from one‐cell zygotes to hatched blastocysts were produced by the application of in vitro maturation, fertilization, and oviductal epithelial cell embryo coculture methods. Transcripts for transforming growth factor (TGF‐α) and platelet‐derived growth factor (PDGF‐A) are detectable in all preimplantation bovine stages as observed in the mouse. Transcripts for TGF‐β2 and insulin‐like growth factor (IGF‐II) and the receptors for PDGF‐α, insulin, IGF‐I, and IGF‐II are also detectable throughout bovine preimplantation development, suggesting that these mRNAs are products of both the maternal and the embryonic genomes in the cow, whereas in the mouse they are present only following the activation of the embryonic genome at the two‐cell stage. In contrast to the mouse embryo, IGF‐I mRNA was detected within preimplantation bovine embryos. Basic fibroblast growth factor (bFGF) is a maternal message in the bovine embryo, since it is only detectable up until the eight‐cell embryo stage. Bovine trophoblast protein (bTP) mRNA was detectable within day 8 bovine blastocysts. As was observed in the mouse, the transcripts for insulin, epidermal growth factor (EGF), or nerve growth factor (NGF) were not detectable in any bovine embryo stage. Analyses of this type should aid the development of a completely defined culture medium for the more efficient production of preimplantation bovine embryos.Keywords
This publication has 58 references indexed in Scilit:
- Co-culture of early cattle embryos to the blastocyst stage with oviducal tissue or in conditioned mediumReproduction, 1989
- Effects of epidermal growth factor on preimplantation mouse embryosReproduction, 1989
- Nucleologenesis and the onset of transcription in the eight‐cell bovine embryo: Fine‐structural autoradiographic studyMolecular Reproduction and Development, 1989
- Autoradiographic evidence for insulin and insulin-like growth factor binding to early mouse embryosDiabetes, 1988
- Nucleolus organizer regions and nucleoli in preattachment bovine embryosReproduction, 1988
- A transcript from a Drosophila pattern gene predicts a protein homologous to the transforming growth factor-β familyNature, 1987
- Stage-specific insulin binding in mouse preimplantation embryosDevelopmental Biology, 1986
- Human insulin receptor and its relationship to the tyrosine kinase family of oncogenesNature, 1985
- Nucleotide sequence of epidermal growth factor cDNA predicts a 128,000-molecular weight protein precursorNature, 1983
- Isolation and nucleotide sequence of a cDNA encoding the precursor of mouse nerve growth factorNature, 1983