Analysis of free carbohydrates in milk using micropacked columns

Abstract

A gas chromatographic method using a micropacked column is described for the analysis of lactose, galactose, lactulose and epilactose in processed milks. The method is evaluated for precision and accuracy using phenyl-β-glucoside as an internal standard. Recoveries near 100% were found for lactulose concentrations higher than 0.1 mgml⁻¹, showing coefficients of variation from 5.9 to 9.4%.