MAML2 rearrangement in Warthin’s tumour: a fluorescent in situ hybridisation study of metaplastic variants
- 15 May 2012
- journal article
- Published by Wiley in Journal of Oral Pathology & Medicine
- Vol. 41 (8), 615-620
- https://doi.org/10.1111/j.1600-0714.2012.01159.x
Abstract
Background: Warthin's tumour (WT) is a common benign lesion of the major salivary glands. The nature of WT remains controversial, with particular regard to the presence of clonal chromosomal abnormalities, including the t(11;19) translocation involving the CRTC1 and MAML2 genes, that have been identified in both WT and mucoepidermoid carcinoma. In this study, we focused our attention on metaplastic WT variants, and we conducted a fluorescent in situ hybridisation (FISH) analysis for the presence of MAML2 gene rearrangement. Methods: Dual-colour FISH analysis was performed on paraffin-embedded sections of eight WTs showing metaplastic changes (five with squamous metaplasia, two with mucinous metaplasia and one with both) using a MAML2 break-apart probe. Results: Presence of split signals indicative of gene rearrangement was identified in a subset of cells in areas of squamous metaplasia in two samples of WT. No rearrangement was observed in the oncocytic epithelium, in lymphocytes and in areas of mucinous metaplasia. Conclusions: The presence of a small subpopulation of cells carrying MAML2 rearrangement in areas of squamous metaplasia within WT could predispose these lesions to malignant transformation in mucoepidermoid carcinoma and could represent a molecular link between the two entities. © 2012 John Wiley & Sons A/SKeywords
This publication has 39 references indexed in Scilit:
- A Reappraisal of the MECT1/MAML2 Translocation in Salivary Mucoepidermoid CarcinomasThe American Journal of Surgical Pathology, 2010
- New insights into the nature of Warthin’s tumourJournal of Oral Pathology & Medicine, 2008
- CRTC1/MAML2 fusion transcript in Warthin's tumor and mucoepidermoid carcinoma: Evidence for a common genetic associationGenes, Chromosomes and Cancer, 2008
- The MECT1-MAML2 Gene Fusion and Benign Warthin's Tumor: Is the MECT1-MAML2 Gene Fusion Specific to Mucuepidermoid Carcinoma?The Journal of Molecular Diagnostics, 2006
- Sustained expression of Mect1-Maml2 is essential for tumor cell growth in salivary gland cancers carrying the t(11;19) translocationOncogene, 2006
- Clinical features of cystadenolymphoma (Warthin’s tumor) of the parotid gland: A retrospective comparative study of 96 casesOral Oncology, 2006
- Parotid Warthin's tumour Bristol Royal Infirmary (1985–1995): a study of histopathology in 33 casesOral Oncology, 2002
- Metaplastic (infarcted) Warthin's tumour of the parotid gland: a possible consequence of fine needle aspiration biopsyHistopathology, 1999
- Cytogenetic characterisation of Warthin's tumourOral Oncology, 1997
- Translocation(11;19)(q14–21;p12) in a parotid mucoepidermoid carcinoma of a childCancer Genetics and Cytogenetics, 1995