Analysis and quantification of flavonoidic compounds from Portuguese olive (Olea Europaea L.) leaf cultivars

Abstract

Twenty three samples of 18 Portuguese olive leaf cultivars were analysed by a reversed-phase HPLC/DAD procedure and eight flavonoidic compounds were identified and quantified (luteolin 7,4′-O-diglucoside, luteolin 7-O-glucoside, rutin, apigenin 7-O-rutinoside, luteolin 4′-O-glucoside, luteolin, apigenin and diosmetin). Luteolin 7,4′-O-diglucoside and luteolin 4′-O-glucoside were identified by HPLC/DAD/MS/MS – ESI. The studied olive leaf samples showed a common phenolic pattern, in which luteolin 4′-O-glucoside was almost always the major compound.