Noninvasive Multimodal Evaluation of Bioengineered Cartilage Constructs Combining Time-Resolved Fluorescence and Ultrasound Imaging
- 1 April 2011
- journal article
- research article
- Published by Mary Ann Liebert Inc in Tissue Engineering, Part C: Methods
- Vol. 17 (4), 495-504
- https://doi.org/10.1089/ten.tec.2010.0368
Abstract
A multimodal diagnostic system that integrates time-resolved fluorescence spectroscopy, fluorescence lifetime imaging microscopy, and ultrasound backscatter microscopy is evaluated here as a potential tool for assessing changes in engineered tissue composition and microstructure nondestructively and noninvasively. The development of techniques capable of monitoring the quality of engineered tissue, determined by extracellular matrix (ECM) content, before implantation would alleviate the need for destructive assays over multiple time points and advance the widespread development and clinical application of engineered tissues. Using a prototype system combining time-resolved fluorescence spectroscopy, FLIM, and UBM, we measured changes in ECM content occurring during chondrogenic differentiation of equine adipose stem cells on 3D biodegradable matrices. The optical and ultrasound results were validated against those acquired via conventional techniques, including collagen II immunohistochemistry, picrosirius red staining, and measurement of construct stiffness. Current results confirm the ability of this multimodal approach to follow the progression of tissue maturation along the chondrogenic lineage by monitoring ECM production (namely, collagen type II) and by detecting resulting changes in mechanical properties of tissue constructs. Although this study was directed toward monitoring chondrogenic tissue maturation, these data demonstrate the feasibility of this approach for multiple applications toward engineering other tissues, including bone and vascular grafts.Keywords
This publication has 46 references indexed in Scilit:
- Comparison of the osteogenic potential of equine mesenchymal stem cells from bone marrow, adipose tissue, umbilical cord blood, and umbilical cord tissueAmerican Journal of Veterinary Research, 2010
- Multigenerational interstitial growth of biological tissuesBiomechanics and Modeling in Mechanobiology, 2010
- Mechanics and mechanobiology of mesenchymal stem cell-based engineered cartilageJournal of Biomechanics, 2010
- Development of a dual-modal tissue diagnostic system combining time-resolved fluorescence spectroscopy and ultrasonic backscatter microscopyReview of Scientific Instruments, 2009
- Nanoindentation of histological specimens: Mapping the elastic properties of soft tissuesJournal of Materials Research, 2009
- Miniaturized side-viewing imaging probe for fluorescence lifetime imaging (FLIM): validation with fluorescence dyes, tissue structural proteins and tissue specimensNew Journal of Physics, 2007
- Laguerre-based method for analysis of time-resolved fluorescence data: application to in-vivo characterization and diagnosis of atherosclerotic lesionsJournal of Biomedical Optics, 2006
- An integral biochemical analysis of the main constituents of articular cartilage, subchondral and trabecular boneOsteoarthritis and Cartilage, 2004
- Discrimination of Human Coronary Artery Atherosclerotic Lipid-Rich Lesions by Time-Resolved Laser-Induced Fluorescence SpectroscopyArteriosclerosis, Thrombosis, and Vascular Biology, 2001
- Identification of hydroxypyridinium crosslinking sites in type II collagen of bovine articular cartilageBiochemistry, 1984