Nanoindentation of histological specimens: Mapping the elastic properties of soft tissues
- 1 March 2009
- journal article
- Published by Springer Science and Business Media LLC in Journal of Materials Research
- Vol. 24 (3), 638-646
- https://doi.org/10.1557/jmr.2009.0130
Abstract
Although alterations in the gross mechanical properties of dynamic and compliant tissues have a major impact on human health and morbidity, there are no well-established techniques to characterize the micromechanical properties of tissues such as blood vessels and lungs. We have used nanoindentation to spatially map the micromechanical properties of 5-μm-thick sections of ferret aorta and vena cava and to relate these mechanical properties to the histological distribution of fluorescent elastic fibers. To decouple the effect of the glass substrate on our analysis of the nanoindentation data, we have used the extended Oliver and Pharr method. The elastic modulus of the aorta decreased progressively from 35 MPa in the adventitial (outermost) layer to 8 MPa at the intimal (innermost) layer. In contrast, the vena cava was relatively stiff, with an elastic modulus >30 MPa in both the extracellular matrix-rich adventitial and intimal regions of the vessel. The central, highly cellularized, medial layer of the vena cava, however, had an invariant elastic modulus of ∼20 MPa. In extracellular matrix-rich regions of the tissue, the elastic modulus, as determined by nanoindentation, was inversely correlated with elastic fiber density. Thus, we show it is possible to distinguish and spatially resolve differences in the micromechanical properties of large arteries and veins, which are related to the tissue microstructure.Keywords
This publication has 48 references indexed in Scilit:
- Poroelastic nanoindentation responses of hydrated boneJournal of Materials Research, 2008
- Effects of Freezing and Cryopreservation on the Mechanical Properties of ArteriesAnnals of Biomedical Engineering, 2006
- Nanoindentation of soft hydrated materials for application to vascular tissuesJournal of Biomedical Materials Research Part A, 2004
- Nanoindentation of bone: Comparison of specimens tested in liquid and embedded in polymethylmethacrylateJournal of Materials Research, 2004
- Fibrillin Microfibrils are Stiff Reinforcing Fibres in Compliant TissuesJournal of Molecular Biology, 2003
- Altered Vascular Function in Young Women with Polycystic Ovary SyndromeJournal of Clinical Endocrinology & Metabolism, 2002
- Aortic Stiffness Is an Independent Predictor of Primary Coronary Events in Hypertensive PatientsHypertension, 2002
- Arbitrary Load Distribution on a Layered Half SpaceJournal of Tribology, 2000
- The effects of hydration on the dynamic mechanical properties of elastinPeptide Science, 1990
- Fluorescence spectra of arterial elastinBiochemical and Biophysical Research Communications, 1967