Molecular Profiling Predicts the Existence of Two Functionally Distinct Classes of Ovarian Cancer Stroma
Open Access
- 9 May 2013
- journal article
- research article
- Published by Hindawi Limited in BioMed Research International
- Vol. 2013, 1-9
- https://doi.org/10.1155/2013/846387
Abstract
Although stromal cell signaling has been shown to play a significant role in the progression of many cancers, relatively little is known about its importance in modulating ovarian cancer development. The purpose of this study was to investigate the process of stroma activation in human ovarian cancer by molecular analysis of matched sets of cancer and surrounding stroma tissues. RNA microarray profiling of 45 tissue samples was carried out using the Affymetrix (U133 Plus 2.0) gene expression platform. Laser capture microdissection (LCM) was employed to isolate cancer cells from the tumors of ovarian cancer patients (Cepi) and matched sets of surrounding cancer stroma (CS). For controls, ovarian surface epithelial cells (OSE) were isolated from the normal (noncancerous) ovaries and normal stroma (NS). Hierarchical clustering of the microarray data resulted in clear separations between the OSE, Cepi, NS, and CS samples. Expression patterns of genes encoding signaling molecules and compatible receptors in the CS and Cepi samples indicate the existence of two subgroups of cancer stroma (CS) with different propensities to support tumor growth. Our results indicate that functionally significant variability exists among ovarian cancer patients in the ability of the microenvironment to modulate cancer development.Keywords
This publication has 30 references indexed in Scilit:
- WNT7A Regulates Tumor Growth and Progression in Ovarian Cancer through the WNT/β-Catenin PathwayMolecular Cancer Research, 2012
- The additional value of TGFβ1 and IL-7 to predict the course of prostate cancer progressionCancer Immunology, Immunotherapy, 2011
- Hallmarks of Cancer: The Next GenerationCell, 2011
- Molecular biology of cancer-associated fibroblasts: Can these cells be targeted in anti-cancer therapy?Seminars in Cell & Developmental Biology, 2010
- Fibroblasts in cancerNature Reviews Cancer, 2006
- TUMOR STROMA AND REGULATION OF CANCER DEVELOPMENTAnnual Review Of Pathology-Mechanisms Of Disease, 2006
- Wnt signalling in stem cells and cancerNature, 2005
- Stromal fibroblasts in cancer initiation and progressionNature, 2004
- Inflammation and cancerNature, 2002
- The Hallmarks of CancerCell, 2000