Sensitization profiles in birch pollen‐allergic patients with and without oral allergy syndrome to apple: lessons from multiplexed component‐resolved allergy diagnosis
- 11 January 2010
- journal article
- Published by Wiley in Clinical and Experimental Allergy
- Vol. 40 (2), 339-347
- https://doi.org/10.1111/j.1365-2222.2009.03345.x
Abstract
Background Component‐resolved diagnosis (CRD) using microarray technology has recently been introduced into the field of clinical allergology. Objective To further validate the use of CRD by microarray technology in allergy diagnosis. Methods Thiry‐seven patients allergic to birch pollen were included. The discriminative value of apple‐specific IgE (sIgE), recombinant Mal d 1 (rMal d 1) sIgE, apple skin prick test and rMal d 1 on the microarray was assessed between patients with a birch‐related oral allergy syndrome to apple (OAS+, n=20) and healthy control individuals (HC, n=8) without a history of inhalant allergies or apple‐induced OAS. An additional comparative analysis was carried out with individuals allergic to birch pollen allergy without OAS (OAS−; n=17). Results rMal d 1 coupled to the microarray constitutes a discriminative marker between OAS+ and HC with a sensitivity 95% and a specificity of 100%. However, in parallel with the traditional sIgE assay, 15 out of 17 OAS− individuals (88%) also displayed IgE reactivity to rMal d 1 coupled to the microarray. OAS− individuals are more frequently sensitized to mite (about three to four times), cat and dog dander (about two to three times) and grass pollen (about 1.5 times) as compared with OAS+ patients. Conclusion At first glance, CRD by microarray seems to be a reliable instrument in the diagnosis of apple‐mediated OAS in birch pollen allergy. However, for discriminating between sensitization and a real allergy, micro‐arrayed rMal d 1 offers no advantage over conventional quantification of rMal d 1 sIgE. Most interestingly, within a single run, birch pollen‐allergic patients without OAS to apple were shown to display a broader sensitization to classical inhalant allergens than birch pollen‐allergic patients with an apple‐related OAS. Cite this as: D. G. Ebo, C. H. Bridts, M. M. Verweij, K. J. De Knop, M. M. Hagendorens, L. S. De Clerck and W. J. Stevens, Clinical & Experimental Allergy, 2010 (40) 339– 347.Keywords
This publication has 15 references indexed in Scilit:
- Clinical usefulness of microarray‐based IgE detection in children with suspected food allergyAllergy, 2008
- Component‐resolved diagnosis in food allergy, are micro‐array assays helpful to the clinician?Allergy, 2008
- Apple allergy across Europe: How allergen sensitization profiles determine the clinical expression of allergies to plant foodsJournal of Allergy and Clinical Immunology, 2006
- Component-resolved diagnostics in food allergyCurrent Opinion in Allergy and Clinical Immunology, 2006
- The performance of a component‐based allergen‐microarray in clinical practiceAllergy, 2006
- Allergy testing: the role of recombinant allergenscclm, 2006
- Flow cytometric analysis of in vitro activated basophils, specific IgE and skin tests in the diagnosis of pollen-associated food allergyCytometry Part B: Clinical Cytometry, 2005
- Protein microarrays for the diagnosis of allergic diseases: state-of-the-art and future developmentcclm, 2005
- Microarrayed allergens for IgE profilingMethods, 2004
- Cloning and Expression of DNA Coding for the Major House Dust Mite Allergen Der p 1 in Escherichia coliInternational Archives of Allergy and Immunology, 1988