Clindamycin Resistance among Staphylococcus Aureus Isolated at Mbarara Regional Referral Hospital, in South Western Uganda
Open Access
- 10 January 2014
- journal article
- Published by Sciencedomain International in British Microbiology Research Journal
- Vol. 4 (12), 1335-1344
- https://doi.org/10.9734/bmrj/2014/10572
Abstract
The study was conducted to determine the prevalence of Clindamycin (CL) resistance and antimicrobial susceptibility among clinical isolates of Staphylococcus aureus (S. aureus) from Mbarara Regional Referral Hospital (MRRH) in Southwestern Uganda. Laboratory based cross sectional study. The study was conducted at the Microbiology department of Mbarara Regional referral hospital between November 2012 and December 2013. In our study, we recruited 300 S. aureus isolates that were stored in the laboratory and were obtained from different clinical samples. The isolates were tested for antimicrobial susceptibility by phenotypic methods and for the genotypic expression of Macrolide Lincosamide StreptograminB (MLSB) resistance genes (ermA, ermB, ermC, and msrA). The D-test was also performed. Phenotypically, a total of 109 (36%) S. aureus isolates were resistant to CL, of which 9 (3%) were constitutively resistant while 100 (33.3%) were inducibly resistant. Genotypicaly, 134/300 (44.7%) isolates possessed at least one of the MLSB resistance genes. 23/300 (7.7%) tested positive for ermB, 98/300 (32.7%) tested positive for the ermC and 43/300 (14.3%) tested positive for the msrA genes with none possessing the ermA gene. Isolates were highly resistant to Sulfamethoxazole/trimethoprim, Erythromycin and Oxacillin with moderate resistance to Vancomycin and Imipenem and least resistance to Linezolid S. aureus resistance to CL was high in this set up. There was also high resistance to Sulfamethoxazole/trimethoprim, Erythromycin and Oxacillin but low resistance to Linezolid.Keywords
This publication has 13 references indexed in Scilit:
- Antibiotic resistance genes & susceptibility patterns in staphylococci2012
- Detection and prevalence of inducible clindamycin resistance in staphylococciJournal of Medical Microbiology, 2007
- Multiplex PCR-based reverse line blot hybridization assay (mPCR/RLB)—a practical epidemiological and diagnostic toolNature Protocols, 2006
- Failure of Clindamycin Treatment of Methicillin-Resistant Staphylococcus aureus Expressing Inducible Clindamycin Resistance In VitroClinical Infectious Diseases, 2003
- Practical Disk Diffusion Method for Detection of Inducible Clindamycin Resistance in Staphylococcus aureus and Coagulase-Negative StaphylococciJournal of Clinical Microbiology, 2003
- Mechanisms of Resistance to Macrolides and Lincosamides: Nature of the Resistance Elements and Their Clinical ImplicationsClinical Infectious Diseases, 2002
- Clindamycin treatment of Staphylococcus aureus expressing inducible clindamycin resistanceJournal of Antimicrobial Chemotherapy, 2001
- Clindamycin, Metronidazole, and ChloramphenicolMayo Clinic Proceedings, 1999
- Emergence of Vancomycin Resistance inStaphylococcus aureusThe New England Journal of Medicine, 1999
- Antibiotic susceptibility testing by a standardized single disk method.1966