OPTICAL STUDIES OF SINGLE MOLECULES AT ROOM TEMPERATURE
- 1 October 1998
- journal article
- Published by Annual Reviews in Annual Review of Physical Chemistry
- Vol. 49 (1), 441-480
- https://doi.org/10.1146/annurev.physchem.49.1.441
Abstract
▪ Abstract Recent developments in optical studies of single molecules at room temperature are reviewed, with an emphasis on the underlying principles and the potential of single-molecule experiments. Examples of single-molecule studies are given, including photophysics and photochemistry pertinent to single-molecule measurements, spectral fluctuations, Raman spectroscopy, diffusional motions, conformational dynamics, fluorescence resonant energy transfer, exciton dynamics, and enzymatic turnovers. These studies illustrate the information obtainable with the single-molecule approach that is hidden in ensemble-averaged measurements.Keywords
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