A scaffold lncRNA shapes the mitosis to meiosis switch
Open Access
- 3 February 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Communications
- Vol. 12 (1), 1-12
- https://doi.org/10.1038/s41467-021-21032-7
Abstract
Long non-coding RNAs (lncRNAs) contribute to the regulation of gene expression in response to intra- or extracellular signals but the underlying molecular mechanisms remain largely unexplored. Here, we identify an uncharacterized lncRNA as a central player in shaping the meiotic gene expression program in fission yeast. We report that this regulatory RNA, termed mamRNA, scaffolds the antagonistic RNA-binding proteins Mmi1 and Mei2 to ensure their reciprocal inhibition and fine tune meiotic mRNA degradation during mitotic growth. Mechanistically, mamRNA allows Mmi1 to target Mei2 for ubiquitin-mediated downregulation, and conversely enables accumulating Mei2 to impede Mmi1 activity, thereby reinforcing the mitosis to meiosis switch. These regulations also occur within a unique Mmi1-containing nuclear body, positioning mamRNA as a spatially-confined sensor of Mei2 levels. Our results thus provide a mechanistic basis for the mutual control of gametogenesis effectors and further expand our vision of the regulatory potential of lncRNAs.Keywords
Funding Information
- Agence Nationale de la Recherche (ANR-16-CE11-0003, ANR-18-CE12-0003, ANR-16-CE12-0031)
- Centre National de la Recherche Scientifique
This publication has 63 references indexed in Scilit:
- Cellular functions of long noncoding RNAsNature, 2019
- Structural insights into the specific recognition of DSR by the YTH domain containing protein Mmi1Biochemical and Biophysical Research Communications, 2017
- Mechanisms of long noncoding RNA function in development and diseaseCellular and Molecular Life Sciences, 2016
- A novel RNA-binding mode of the YTH domain reveals the mechanism for recognition of determinant of selective removal by Mmi1Nucleic Acids Research, 2015
- Regulation of mRNA Levels by Decay-Promoting Introns that Recruit the Exosome Specificity Factor Mmi1Cell Reports, 2015
- Hexanucleotide motifs mediate recruitment of the RNA elimination machinery to silent meiotic genesOpen Biology, 2012
- Long noncoding RNAs and human diseaseTrends in Cell Biology, 2011
- The selective elimination of messenger RNA underlies the mitosis-meiosis switch in fission yeastProceedings of the Japan Academy, Series B, 2010
- Transcriptional regulatory network for sexual differentiation in fission yeastGenome Biology, 2007
- Selective elimination of messenger RNA prevents an incidence of untimely meiosisNature, 2006