Hexanucleotide motifs mediate recruitment of the RNA elimination machinery to silent meiotic genes
Open Access
- 1 March 2012
- journal article
- research article
- Published by The Royal Society in Open Biology
- Vol. 2 (3), 120014
- https://doi.org/10.1098/rsob.120014
Abstract
The selective elimination system blocks the accumulation of meiosis-specific mRNAs during the mitotic cell cycle in fission yeast. These mRNAs harbour a region, the determinant of selective removal (DSR), which is recognized by a YTH-family RNA-binding protein, Mmi1. Mmi1 directs target transcripts to destruction in association with nuclear exosomes. Hence, the interaction between DSR and Mmi1 is crucial to discriminate mitosis from meiosis. Here, we show that Mmi1 interacts with repeats of the hexanucleotide U(U/C)AAAC that are enriched in the DSR. Disruption of this ‘DSR core motif’ in a target mRNA inhibits its elimination. Tandem repeats of the motif can function as an artificial DSR. Mmi1 binds to it in vitro . Thus, a core motif cluster is responsible for the DSR activity. Furthermore, certain variant hexanucleotide motifs can augment the function of the DSR core motif. Notably, meiRNA, which composes the nuclear Mei2 dot required to suppress Mmi1 activity during meiosis, carries numerous copies of the core/augmenting motifs on its tail and is indeed degraded by the Mmi1/exosome system, indicating its likely role as decoy bait for Mmi1.Keywords
This publication has 31 references indexed in Scilit:
- Mmi1 RNA surveillance machinery directs RNAi complex RITS to specific meiotic genes in fission yeastThe EMBO Journal, 2012
- RNA Elimination Machinery Targeting Meiotic mRNAs Promotes Facultative Heterochromatin FormationScience, 2012
- The Fission Yeast RNA Binding Protein Mmi1 Regulates Meiotic Genes by Controlling Intron Specific Splicing and Polyadenylation Coupled RNA TurnoverPLOS ONE, 2011
- Multiplex preamplification PCR and microsatellite validation enables accurate single nucleotide polymorphism genotyping of historical fish scalesMolecular Ecology Resources, 2011
- Red1 promotes the elimination of meiosis-specific mRNAs in vegetatively growing fission yeastThe EMBO Journal, 2011
- Importance of polyadenylation in the selective elimination of meiotic mRNAs in growing S. pombe cellsThe EMBO Journal, 2010
- The YTH Domain Is a Novel RNA Binding DomainJournal of Biological Chemistry, 2010
- The transcriptional program of meiosis and sporulation in fission yeastNature Genetics, 2002
- A Gibbs Sampling Method to Detect Overrepresented Motifs in the Upstream Regions of Coexpressed GenesJournal of Computational Biology, 2002
- The Interaction and Colocalization of Sam68 with the Splicing-associated Factor YT521-B in Nuclear Dots Is Regulated by the Src Family Kinase p59fynMolecular Biology of the Cell, 1999