One-dimensional topography underlies three-dimensional fibrillar cell migration
Open Access
- 16 February 2009
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 184 (4), 481-490
- https://doi.org/10.1083/jcb.200810041
Abstract
Current concepts of cell migration were established in regular two-dimensional (2D) cell culture, but the roles of topography are poorly understood for cells migrating in an oriented 3D fibrillar extracellular matrix (ECM). We use a novel micropatterning technique termed microphotopatterning (μPP) to identify functions for 1D fibrillar patterns in 3D cell migration. In striking contrast to 2D, cell migration in both 1D and 3D is rapid, uniaxial, independent of ECM ligand density, and dependent on myosin II contractility and microtubules (MTs). 1D and 3D migration are also characterized by an anterior MT bundle with a posterior centrosome. We propose that cells migrate rapidly through 3D fibrillar matrices by a 1D migratory mechanism not mimicked by 2D matrices.Keywords
This publication has 27 references indexed in Scilit:
- Contact Guidance Mediated Three-Dimensional Cell Migration is Regulated by Rho/ROCK-Dependent Matrix ReorganizationBiophysical Journal, 2008
- In migrating cells, the Golgi complex and the position of the centrosome depend on geometrical constraints of the substratumJournal of Cell Science, 2008
- Of Extracellular Matrix, Scaffolds, and Signaling: Tissue Architecture Regulates Development, Homeostasis, and CancerAnnual Review of Cell and Developmental Biology, 2006
- Migration of tumor cells in 3D matrices is governed by matrix stiffness along with cell-matrix adhesion and proteolysisProceedings of the National Academy of Sciences of the United States of America, 2006
- A Rac switch regulates random versus directionally persistent cell migrationThe Journal of cell biology, 2005
- Cell Migration: Integrating Signals from Front to BackScience, 2003
- Nanofabrication for micropatterned cell arrays by combining electron beam‐irradiated polymer grafting and localized laser ablationJournal of Biomedical Materials Research Part A, 2003
- Experimental manipulation of a contact guidance system in amphibian gastrulation by mechanical tensionNature, 1984
- Mechanism of retraction of the trailing edge during fibroblast movement.The Journal of cell biology, 1981
- Induction of spreading during fibroblast movement.The Journal of cell biology, 1979