Restriction site polymorphisms in the genes encoding new members of group 3 outer membrane protein family ofBrucellaspp.
Open Access
- 1 April 2005
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 245 (1), 79-84
- https://doi.org/10.1016/j.femsle.2005.02.026
Abstract
Thirty-seven Brucella reference and field strains representing all the species and their biovars were analysed by PCR–RFLP to determine the degree of variation in the genes encoding the new members of group 3 outer membrane protein (Omp) family. Analysis of the omp22 and omp25c/omp25d genes indicated that the restriction patterns were identical for all species and biovars with all restriction enzymes tested, except for Brucella ovis that showed a short 30 bp deletion close to omp22 gene, and for B. abortus biovar 6 and B. ovis that lacked a DdeI site and a HinfI site, respectively, in the omp25c/omp25d genes. Analysis of PCR products of the omp31b gene digested with 20 restriction enzymes revealed that this gene has a greater level of DNA polymorphism than the other genes encoding the new members of group 3 Omp family. A deletion of 232 bp was detected in fourteen B melitensis strains from different hosts and from different geographic origins, confirming that this feature is indeed a hallmark of B. melitensis. PCR–RFLP analysis of omp31b with DdeI allowed us to identify species-specific markers for B. abortus, B. melitensis, and B. ovis. Finally, by PCR analysis, Southern blot hybridization and DNA sequencing we showed that a large deletion of 15 kb, comprising the entire omp25b gene and 21 more genes, is present in all B. ovis strains, thus confirming previous observations from other authors.Keywords
This publication has 16 references indexed in Scilit:
- Comparative Whole-Genome Hybridization Reveals Genomic Islands inBrucellaSpeciesJournal of Bacteriology, 2004
- Characterization of New Members of the Group 3 Outer Membrane Protein Family of Brucella sppInfection and Immunity, 2003
- The two-component system BvrR/BvrS essential for Brucella abortus virulence regulates the expression of outer membrane proteins with counterparts in members of the RhizobiaceaeProceedings of the National Academy of Sciences of the United States of America, 2002
- Global analysis of the Brucella melitensis proteome: Identification of proteins expressed in laboratory-grown cultureProteomics, 2002
- The genome sequence of the facultative intracellular pathogen Brucella melitensisProceedings of the National Academy of Sciences of the United States of America, 2001
- DNA polymorphism at the omp-31 locus of Brucella spp.: evidence for a large deletion in Brucella abortus, and other species-specific markersMicrobiology, 1997
- Brucellosis: an OverviewEmerging Infectious Diseases, 1997
- Restriction site polymorphism of the genes encoding the major 25 kDa and 36 kDa outer-membrane proteins of BrucellaMicrobiology, 1995
- An Overview of Human BrucellosisClinical Infectious Diseases, 1995
- Genetic variation at the omp2 porin locus of the brucellae: species‐specific markersMolecular Microbiology, 1990