Heterogeneity of mesenchymal stromal cell preparations
- 1 January 2008
- journal article
- review article
- Published by Elsevier BV in Cytotherapy
- Vol. 10 (4), 320-330
- https://doi.org/10.1080/14653240802217011
Abstract
As an archetype of human adult stem cells that can readily be harvested, enriched and expanded in vitro, mesenchymal stromal cells (MSC) have been reported to be of significance for regenerative medicine. The literature is replete with reports on their developmental potentials in pre-clinical model systems. Different preparative protocols have been shown to yield MSC-like cell cultures or even cell lines, from starting materials as diverse as bone marrow, fat tissue, fetal cord blood and peripheral blood. However, MSC are still ill-defined by physical, phenotypic and functional properties. The quality of preparations from different laboratories varies tremendously and the cell products are notoriously heterogeneous. The source and freshness of the starting material, culture media used, presence of animal sera, cytokines, cell density, number of passages upon culture, etc., all have a significant impact on the (1) cell type components and heterogeneity of the initial population, (2) differential expansion of specific subsets, with different potentials of the end products, and (3) long-term functional fate of MSC as well as other types of progenitor cells that are co-cultivated with them. Consequently, there is an urgent need for the development of reliable reagents, common guidelines and standards for MSC preparations and of precise molecular and cellular markers to define subpopulations with diverse pathways of differentiation and divergent potentials.Keywords
This publication has 85 references indexed in Scilit:
- Replicative Senescence of Mesenchymal Stem Cells: A Continuous and Organized ProcessPLOS ONE, 2008
- Processus and recessus adhaerentes: giant adherens cell junction systems connect and attract human mesenchymal stem cellsCell and tissue research, 2007
- Hematopoietic reconstitution by multipotent adult progenitor cells: precursors to long-term hematopoietic stem cellsThe Journal of Experimental Medicine, 2007
- Proliferation and differentiation of bone marrow stromal cells under hypoxic conditionsBiochemical and Biophysical Research Communications, 2006
- Comparative Analysis of Mesenchymal Stem Cells from Bone Marrow, Umbilical Cord Blood, or Adipose TissueThe International Journal of Cell Cloning, 2006
- Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statementCytotherapy, 2006
- Differential Expression of Novel Potential Regulators in Hematopoietic Stem CellsPLoS Genetics, 2005
- Pluripotency of mesenchymal stem cells derived from adult marrowNature, 2002
- Multilineage Cells from Human Adipose Tissue: Implications for Cell-Based TherapiesTissue Engineering, 2001
- Turning Blood into Brain: Cells Bearing Neuronal Antigens Generated in Vivo from Bone MarrowScience, 2000