Long-Term Proliferation in Culture and Germline Transmission of Mouse Male Germline Stem Cells1
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- 1 August 2003
- journal article
- Published by Oxford University Press (OUP) in Biology of Reproduction
- Vol. 69 (2), 612-616
- https://doi.org/10.1095/biolreprod.103.017012
Abstract
Spermatogenesis is a complex process that originates in a small population of spermatogonial stem cells. Here we report the in vitro culture of spermatogonial stem cells that proliferate for long periods of time. In the presence of glial cell line-derived neurotrophic factor, epidermal growth factor, basic fibroblast growth factor, and leukemia inhibitory factor, gonocytes isolated from neonatal mouse testis proliferated over a 5-month period (>10(14)-fold) and restored fertility to congenitally infertile recipient mice following transplantation into seminiferous tubules. Long-term spermatogonial stem cell culture will be useful for studying spermatogenesis mechanism and has important implications for developing new technology in transgenesis or medicine.Keywords
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