Transport, Manipulation, and Reaction of Biological Cells On-Chip Using Electrokinetic Effects
- 1 April 1997
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 69 (8), 1564-1568
- https://doi.org/10.1021/ac9606564
Abstract
A microfluidic system was fabricated on a glass chip to study mobilization of biological cells on-chip. Electroosmotic and/or electrophoretic pumping were used to drive the cell transport within a network of capillary channels. Whole cells such as Saccharomyces cerevisiae, canine erythrocyte, and Escherichia coli were employed in this work. Photographs are presented to illustrate how cells are selected and transported from one location to another within the capillary network, with velocities up to about 0.5 mm/s in capillaries with a 15- × 55-μm cross section. The mixing of canine erythrocytes with the lysing agent sodium dodecyl sulfate, at an intersection within the chip, was performed to demonstrate that cell selection and subsequent reaction can be accomplished within the microchip.Keywords
This publication has 16 references indexed in Scilit:
- Separation of hemoglobin variants in single human erythrocytes by capillary electrophoresis with laser-induced native fluorescence detectionJournal of Chromatography A, 1995
- Manipulation of a single cell with microcapillary tubing based on its electrophoretic mobilityElectrophoresis, 1995
- Electroosmotic Pumping and Valveless Control of Fluid Flow within a Manifold of Capillaries on a Glass ChipAnalytical Chemistry, 1994
- Normal cations and abnormal membrane lipids in the red blood cells of dogs with familial stomatocytosis-hypertrophic gastritisBlood, 1994
- Electrochemical detection in microcolumn separationsAnalytical Chemistry, 1994
- Glass chips for high-speed capillary electrophoresis separations with submicrometer plate heightsAnalytical Chemistry, 1993
- Micromachining a Miniaturized Capillary Electrophoresis-Based Chemical Analysis System on a ChipScience, 1993
- Planar glass chips for capillary electrophoresis: repetitive sample injection, quantitation, and separation efficiencyAnalytical Chemistry, 1993
- Capillary electrophoresis and sample injection systems integrated on a planar glass chipAnalytical Chemistry, 1992
- Flow-injection analysis for electroinactive anions at a polyaniline electrodeAnalytical Chemistry, 1988