Orthopedic implant cobalt‐alloy particles produce greater toxicity and inflammatory cytokines than titanium alloy and zirconium alloy‐based particlesin vitro, in human osteoblasts, fibroblasts, and macrophages
- 21 May 2012
- journal article
- research article
- Published by Wiley in Journal of Biomedical Materials Research Part A
- Vol. 100A (8), 2147-2158
- https://doi.org/10.1002/jbm.a.34122
Abstract
The performance of total joint arthroplasty (TJA) depends on the size/shape, material, and amounts of implant debris. Much remains unknown in terms of which types of debris are most reactive. We compared the responses of human periimplant cells, osteoblasts, fibroblasts, and macrophages, exposed to particles of different metal-based particles (i.e., cobalt-chromium-molybdenum (CoCrMo) alloy, titanium (Ti) alloy, zirconium (Zr) oxide, and Zr alloy. CoCrMo-alloy particles were by far the most toxic (p < 0.05) and decreased viability and proliferation of human osteoblasts, fibroblasts, and macrophages by >50% at a dose of only 50 particles per cell. All particle types induced the production of interleukin (IL)-6, tumor necrosis factor (TNF)-α, and IL-8 by osteoblasts, fibroblasts, and monocytes/macrophages. However, the greatest cytokine responses of macrophages were to CoCrMo alloy (TNF-α and IL-8) and Ti alloy (IL-1β). Likewise, the greatest responses of fibroblasts and osteoblasts were to CoCrMo alloy (IL-6 and TNF-α) (i.e., IL-6 300 pg/mL; 30-fold max, TNF-α 150 pg/mL; 15-fold max) versus controls. For macrophages, CoCrMo particles induced IL-8 (> 2000 pg/mL; approx 100-fold max) above controls and were also significantly elevated above levels produced by Zr-based particles. Submicron sized (0.2-0.9 μm) Zr-based particles (originally presumed to be more reactive) induced less toxicity and inflammatory responses when compared with larger (approx 1 μm) CoCrMo-alloy and Ti-alloy particles. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.This publication has 78 references indexed in Scilit:
- Micrometer-Sized Titanium Particles Can Induce Potent Th2-Type Responses through TLR4-Independent PathwaysThe Journal of Immunology, 2011
- Immunologic Adverse Reaction Associated with Low-carbide Metal-on-metal Bearings in Total Hip ArthroplastyClinical Orthopaedics and Related Research, 2010
- Co–Cr–Mo alloy particles induce tumor necrosis factor alpha production in MLO-Y4 osteocytes: A role for osteocytes in particle-induced inflammationBone, 2009
- Soluble and particulate Co‐Cr‐Mo alloy implant metals activate the inflammasome danger signaling pathway in human macrophages: A novel mechanism for implant debris reactivityJournal of Orthopaedic Research, 2008
- Silica crystals and aluminum salts activate the NALP3 inflammasome through phagosomal destabilizationNature Immunology, 2008
- Th1 type lymphocyte reactivity to metals in patients with total hip arthroplastyJournal of Orthopaedic Surgery and Research, 2008
- Wear particles, periprosthetic osteolysis and the immune systemBiomaterials, 2007
- Complications and reoperations of the SB Charité lumbar disc prosthesis: experience in 75 patientsEuropean Spine Journal, 2007
- Aseptic looseningGene Therapy, 2004
- In vitro activation of human fibroblasts by retrieved titanium alloy wear debrisJournal of Orthopaedic Research, 1996