Immobilization of Ferrocene-Modified SNAP-Fusion Proteins
Open Access
- 18 February 2013
- journal article
- research article
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 14 (2), 4066-4080
- https://doi.org/10.3390/ijms14024066
Abstract
The supramolecular assembly of proteins on surfaces has been investigated via the site-selective incorporation of a supramolecular moiety on proteins. To this end, fluorescent proteins have been site-selectively labeled with ferrocenes, as supramolecular guest moieties, via SNAP-tag technology. The assembly of guest-functionalized SNAP-fusion proteins on cyclodextrin- and cucurbit[7]uril-coated surfaces yielded stable monolayers. The binding of all ferrocene fusion proteins is specific as determined by surface plasmon resonance. Micropatterns of the fusion proteins, on patterned cyclodextrin and cucurbituril surfaces, have been visualized using fluorescence microscopy. The SNAP-fusion proteins were also immobilized on cyclodextrin vesicles. The supramolecular SNAP-tag labeling of proteins, thus, allows for the assembly of modified proteins via supramolecular host-guest interaction on different surfaces in a controlled manner. These findings extend the toolbox of fabricating supramolecular protein patterns on surfaces taking advantage of the high labeling efficiency of the SNAP-tag with versatile supramolecular moieties.This publication has 36 references indexed in Scilit:
- Supramolecularly Oriented Immobilization of Proteins Using Cucurbit[8]urilLangmuir, 2012
- Reversible and Oriented Immobilization of Ferrocene-Modified ProteinsJournal of the American Chemical Society, 2012
- Applications of Protein Biochips in Biomedical and Biotechnological ResearchAngewandte Chemie-International Edition, 2009
- Selective Covalent Protein Immobilization: Strategies and ApplicationsChemical Reviews, 2009
- Surface Functionalization for Protein and Cell PatterningPublished by Springer Science and Business Media LLC ,2009
- Understanding enzyme immobilisationChemical Society Reviews, 2008
- Nanometer Arrays of Functional Light Harvesting Antenna Complexes by Nanoimprint Lithography and Host−Guest InteractionsJournal of the American Chemical Society, 2008
- Assembly of Bionanostructures onto β-Cyclodextrin Molecular Printboards for Antibody Recognition and Lymphocyte Cell CountingJournal of the American Chemical Society, 2008
- Anchoring of Histidine‐Tagged Proteins to Molecular Printboards: Self‐assembly, Thermodynamic Modeling, and PatterningChemistry – A European Journal, 2008
- Noncovalent Immobilization of Proteins on a Solid Surface by Cucurbit[7]uril-Ferrocenemethylammonium Pair, a Potential Replacement of Biotin−Avidin PairJournal of the American Chemical Society, 2007