Anchoring of Histidine‐Tagged Proteins to Molecular Printboards: Self‐assembly, Thermodynamic Modeling, and Patterning
- 21 February 2008
- journal article
- research article
- Published by Wiley in Chemistry – A European Journal
- Vol. 14 (7), 2044-2051
- https://doi.org/10.1002/chem.200701478
Abstract
In this paper the multivalent binding of hexahistidine (His6)-tagged proteins to β-cyclodextrin (β-CD) self-assembled monolayers (SAMs) by using the nickel(II) complex of a hetero-divalent orthogonal adamantyl nitrilotriacetate linker (4) is described. Nonspecific interactions were suppressed by using monovalent adamantyl-hexa(ethylene glycol) derivative 3. With the mono-His6-tagged maltose binding protein (His6-MBP), thermodynamic modeling based on surface plasmon resonance (SPR) titration data showed that the MBP molecules in solution were linked, on average, to Ni⋅4 in 1:1 stoichiometry. On the surface, however, the majority of His6-MBP was complexed to surface-immobilized β-CDs through three Ni⋅4 complexes. This difference is explained by the high effective β-CD concentration at the surface and is a new example of supramolecular interfacial expression. In a similar adsorption scheme, SPR proved that the α-proteasome could be attached to β-CD SAMs in a specific manner. Patterning through microcontact printing of (His6)4-DsRed-fluorescent timer (DsRed-FT), which is a tetrameric, visible autofluorescent protein, was carried out in the presence of Ni⋅4. Fluorescence measurements showed that the (His6)4-DsRed-FT is bound strongly through Ni⋅4 to the molecular printboard.Keywords
This publication has 56 references indexed in Scilit:
- Glucose biosensor based on the layer-by-layer self-assembling of glucose oxidase and chitosan derivatives on a thiolated gold surfaceAnalytica Chimica Acta, 2006
- Immobilization of Histidine-Tagged Recombinant Proteins onto Micropatterned Surfaces for Cell-Based Functional AssaysLangmuir, 2005
- Supramolecular Microcontact Printing and Dip‐Pen Nanolithography on Molecular PrintboardsChemistry – A European Journal, 2005
- Electrogeneration of a Poly(pyrrole)-NTA Chelator Film for a Reversible Oriented Immobilization of Histidine-Tagged ProteinsJournal of the American Chemical Society, 2005
- Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent proteinNature Biotechnology, 2004
- Single-Step Formation of a Biorecognition Layer for Assaying Histidine-Tagged ProteinsAnalytical Chemistry, 2004
- Oriented Binding of the His6-Tagged Carboxyl-Tail of the L-type Ca2+ Channel α1-Subunit to a New NTA-Functionalized Self-Assembled MonolayerLangmuir, 2004
- The molecular properties and applications of Anthozoa fluorescent proteins and chromoproteinsNature Biotechnology, 2004
- High-resolution AFM-imaging and mechanistic analysis of the 20 S proteasomeJournal of Molecular Biology, 1999
- Polyvalent Interactions in Biological Systems: Implications for Design and Use of Multivalent Ligands and InhibitorsAngewandte Chemie-International Edition, 1998