Cross-Feeding between Bifidobacterium longum BB536 and Acetate-Converting, Butyrate-Producing Colon Bacteria during Growth on Oligofructose
- 1 December 2006
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 72 (12), 7835-7841
- https://doi.org/10.1128/aem.01296-06
Abstract
In vitro coculture fermentations of Bifidobacterium longum BB536 and two acetate-converting, butyrate-producing colon bacteria, Anaerostipes caccae DSM 14662 and Roseburia intestinalis DSM 14610, with oligofructose as the sole energy source, were performed to study interspecies interactions. Two clearly distinct types of cross-feeding were identified. A. caccae DSM 14662 was not able to degrade oligofructose but could grow on the fructose released by B. longum BB536 during oligofructose breakdown. R. intestinalis DSM 14610 could degrade oligofructose, but only after acetate was added to the medium. Detailed kinetic analyses of oligofructose breakdown by the last strain revealed simultaneous degradation of the different chain length fractions, in contrast with the preferential degradation of shorter fractions by B. longum BB536. In a coculture of both strains, initial oligofructose degradation and acetate production by B. longum BB536 took place, which in turn also allowed oligofructose breakdown by R. intestinalis DSM 14610. These and similar cross-feeding mechanisms could play a role in the colon ecosystem and contribute to the combined bifidogenic/butyrogenic effect observed after addition of inulin-type fructans to the diet.Keywords
This publication has 62 references indexed in Scilit:
- Kinetic Analysis of Bifidobacterial Metabolism Reveals a Minor Role for Succinic Acid in the Regeneration of NAD + through Its Growth-Associated ProductionApplied and Environmental Microbiology, 2006
- Two Routes of Metabolic Cross-Feeding between Bifidobacterium adolescentis and Butyrate-Producing Anaerobes from the Human GutApplied and Environmental Microbiology, 2006
- Lactate-Utilizing Bacteria, Isolated from Human Feces, That Produce Butyrate as a Major Fermentation ProductApplied and Environmental Microbiology, 2004
- Inulin and oligofructose modulate lipid metabolism in animals: review of biochemical events and future prospectsBritish Journal of Nutrition, 2002
- Prebiotic effects of inulin and oligofructoseBritish Journal of Nutrition, 2002
- Inulin and oligofructose in the dietary fibre conceptBritish Journal of Nutrition, 2002
- Fermentation and bulking capacity of indigestible carbohydrates: the case of inulin and oligofructoseBritish Journal of Nutrition, 2002
- Butyrate inhibits colon carcinoma cell growth through two distinct pathwaysSurgery, 1998
- Antagonistic effects of sulfide and butyrate on proliferation of colonic mucosaDigestive Diseases and Sciences, 1996
- Effects of the in vitro fermentation of oligofructose and inulin by bacteria growing in the human large intestineJournal of Applied Bacteriology, 1993