Kinetic Analysis of Bifidobacterial Metabolism Reveals a Minor Role for Succinic Acid in the Regeneration of NAD + through Its Growth-Associated Production
- 1 August 2006
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 72 (8), 5204-5210
- https://doi.org/10.1128/aem.00146-06
Abstract
Several strains belonging to the genus Bifidobacterium were tested to determine their abilities to produce succinic acid. Bifidobacterium longum strain BB536 and Bifidobacterium animalis subsp. lactis strain Bb 12 were kinetically analyzed in detail using in vitro fermentations to obtain more insight into the metabolism and production of succinic acid by bifidobacteria. Changes in end product formation in strains of Bifidobacterium could be related to the specific rate of sugar consumption. When the specific sugar consumption rate increased, relatively more lactic acid and less acetic acid, formic acid, and ethanol were produced, and vice versa. All Bifidobacterium strains tested produced small amounts of succinic acid; the concentrations were not more than a few millimolar. Succinic acid production was found to be associated with growth and stopped when the energy source was depleted. The production of succinic acid contributed to regeneration of a small part of the NAD + , in addition to the regeneration through the production of lactic acid and ethanol.Keywords
This publication has 42 references indexed in Scilit:
- A Bile Salt-Resistant Derivative of Bifidobacterium animalis Has an Altered Fermentation Pattern When Grown on Glucose and MaltoseApplied and Environmental Microbiology, 2005
- Overview on sugar metabolism and its control in Lactococcus lactis – The input from in vivo NMRFEMS Microbiology Reviews, 2005
- Lessons from the genomes of bifidobacteriaFEMS Microbiology Reviews, 2005
- Overview on sugar metabolism and its control inLactococcus lactis— The input from in vivo NMRFEMS Microbiology Reviews, 2005
- Lessons from the genomes of bifidobacteriaFEMS Microbiology Reviews, 2005
- Getting better with bifidobacteriaJournal of Applied Microbiology, 2005
- Lactate-Utilizing Bacteria, Isolated from Human Feces, That Produce Butyrate as a Major Fermentation ProductApplied and Environmental Microbiology, 2004
- Bifidobacterium longum Requires a Fructokinase (Frk; ATP: d -Fructose 6-Phosphotransferase, EC 2.7.1.4) for Fructose CatabolismJournal of Bacteriology, 2004
- The Pool of ADP and ATP Regulates Anaerobic Product Formation in Resting Cells of Lactococcus lactisApplied and Environmental Microbiology, 2004
- Acetate Utilization and Butyryl Coenzyme A (CoA):Acetate-CoA Transferase in Butyrate-Producing Bacteria from the Human Large IntestineApplied and Environmental Microbiology, 2002