Photoswitchable Nanoprobes Offer Unlimited Brightness in Frequency-Domain Imaging
- 3 May 2011
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 133 (20), 7628-7631
- https://doi.org/10.1021/ja1108479
Abstract
A single probe has limited brightness in time-domain imaging and such limitation frequently renders individual molecules undetectable in the presence of interference or complex cellular structures. However, a single photoswitchable probe produces a signal, which can be separated from interference or noise using photoswitching-enabled Fourier transformation (PFT). As a result, the light-modulated probes can be made super bright in the frequency domain simply by acquiring more cycles in the time domain.Keywords
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