Separating human DNA mixtures using denaturing high-performance liquid chromatography
- 9 January 2005
- journal article
- review article
- Published by Taylor & Francis Ltd in Expert Review of Molecular Diagnostics
- Vol. 5 (1), 53-63
- https://doi.org/10.1586/14737159.5.1.53
Abstract
DNA mixtures represent challenging samples that are rarely amenable to direct DNA sequence analysis and many of the strategies available to separate mixtures are both labor and time intensive. Denaturing high-performance liquid chromatography is an accurate and rapid approach for the detection and scoring of mutations. It can also be used to separate DNA mixtures. The technique relies on the chromatographic separation of crosshybridization products to isolate the individual components of a mixture. By eliminating secondary amplification and excessive manipulation prior to sequencing, denaturing high-performance liquid chromatography can streamline the analysis of conditions ranging from somatic mosaicism, microchimerism and mitochondrial heteroplasmy to evidentiary material containing mixtures of DNA encountered in forensic investigations.Keywords
This publication has 48 references indexed in Scilit:
- Detection of microchimerism by PCR is a function of amplification strategyTransfusion, 2001
- High Rate of Mosaicism in Tuberous Sclerosis ComplexAmerican Journal of Human Genetics, 1999
- Detection of Single-Nucleotide Polymorphisms with the WAVE™ DNA Fragment Analysis SystemGenetic Testing, 1997
- Rapid and enhanced detection of mitochondrial DNA variation using single-strand conformation analysis of superposed restriction enzyme fragments from polymerase chain reaction-amplified productsElectrophoresis, 1997
- Screening for human mitochondrial DNA polymorphisms with denaturing gradient gel electrophoresisHuman Genetics, 1996
- Mitochondrial DNA sequence heteroplasmy in the Grand Duke of Russia Georgij Romanov establishes the authenticity of the remains of Tsar Nicholas IINature Genetics, 1996
- Rapid detection of sequence polymorphisms in the human mitochondrial DNA control region by polymerase chain reaction and single‐strand conformation analysis in mutation detection enhancement gelsElectrophoresis, 1996
- Direct sequence analysis of TCR Vδ2–Dδ3 rearrangements in common acute lymphoblastic leukaemia and application to detection of minimal residual diseaseBritish Journal of Haematology, 1993
- Identification of the skeletal remains of a murder victim by DNA analysisNature, 1991
- Sequence and organization of the human mitochondrial genomeNature, 1981