Methods developed for SELEX
- 28 October 2006
- journal article
- review article
- Published by Springer Science and Business Media LLC in Analytical and Bioanalytical Chemistry
- Vol. 387 (1), 171-182
- https://doi.org/10.1007/s00216-006-0826-2
Abstract
SELEX (systematic evolution of ligands by exponential enrichment) is a process that involves the progressive purification from a combinatorial library of nucleic acid ligands with a high affinity for a particular target by repeated rounds of partitioning and amplification. With the development of aptamer technology over the last decade, various modified SELEX processes have arisen that allow various aptamers to be developed against a wide variety of molecules, irrespective of the target size. In the present review, the separation methods used in such SELEX processes are reviewed.Keywords
This publication has 103 references indexed in Scilit:
- Selection and Stabilization of the RNA Aptamers against the Human Immunodeficiency Virus Type-1 Nucleocapsid ProteinBiochemical and Biophysical Research Communications, 2002
- In vitro selection of preferred DNA pairing sequences by the Escherichia coli RecA protein.Genes & Development, 1996
- In vitro selection of RNA-based irreversible inhibitors of human neutrophil elastaseCell Chemical Biology, 1995
- In Vitro Selection of RNA Ligands to Substance PBiochemistry, 1995
- RNA Selection: Aptamers achieve the desired recognitionCurrent Biology, 1994
- Selection of high affinity RNA ligands to the bacteriophage R17 coat proteinJournal of Molecular Biology, 1992
- In vitro selection of RNA molecules that bind specific ligandsNature, 1990
- Systematic Evolution of Ligands by Exponential Enrichment: RNA Ligands to Bacteriophage T4 DNA PolymeraseScience, 1990
- Sequence-specific interaction of R17 coat protein with its ribonucleic acid binding siteBiochemistry, 1983
- Evolution in vitro: Sequence and phenotype of a mutant RNA resistant to ethidium bromideJournal of Molecular Biology, 1974