Luminescent visualization of antigens on blots
- 27 September 1986
- journal article
- research article
- Published by Elsevier BV in Journal of Immunological Methods
- Vol. 92 (2), 161-165
- https://doi.org/10.1016/0022-1759(86)90161-4
Abstract
Proteins separated by SDS gel electrophoresis and transferred to a nitrocellulose sheet can be visualised by 'probing' with peroxidase-linked reagents which are detected by luminescence. A modified luminescent substrate is described containing 4-methylumbelliferone which enhances light emission four-fold. Using the modified substrate, luminescent detection was found to be more sensitive than chromogenic detection of peroxidase using 4-chloro-1-napthol. The new technique was used in conjunction with the immunoblot method to demonstrate antigenic differences between rat and mouse erythrocytes.Keywords
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