Gene Regulation with Polyvalent siRNA−Nanoparticle Conjugates
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- 26 January 2009
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 131 (6), 2072-2073
- https://doi.org/10.1021/ja808719p
Abstract
We report the synthesis and characterization of polyvalent RNA−gold nanoparticle conjugates (RNA−Au NPs), nanoparticles that are densely functionalized with synthetic RNA oligonucleotides and designed to function in the RNAi pathway. The particles were rationally designed and synthesized to be free of degrading enzymes, have a high surface loading of siRNA duplexes, and contain an auxiliary passivating agent for increased stability in biological media. The resultant conjugates have a half-life six times longer than that of free dsRNA, readily enter cells without the use of transfection agents, and demonstrate a high gene knockdown capability in a cell model.Keywords
This publication has 29 references indexed in Scilit:
- Nano-Flares: Probes for Transfection and mRNA Detection in Living CellsJournal of the American Chemical Society, 2007
- Specific and sensitive detection of nucleic acids and RNases using gold nanoparticle–RNA–fluorescent dye conjugatesChemical Communications, 2007
- Maximizing DNA Loading on a Range of Gold Nanoparticle SizesAnalytical Chemistry, 2006
- Smart PEGylated Gold Nanoparticles for the Cytoplasmic Delivery of siRNA to Induce Enhanced Gene SilencingChemistry Letters, 2006
- Engineering the nanoparticle–biomacromolecule interfaceSoft Matter, 2006
- Controllable Self-Assembly of Nanoparticles for Specific Delivery of Multiple Therapeutic Molecules to Cancer Cells Using RNA NanotechnologyNano Letters, 2005
- A Thermodynamic Investigation into the Binding Properties of DNA Functionalized Gold Nanoparticle Probes and Molecular Fluorophore ProbesJournal of the American Chemical Society, 2005
- What Controls the Melting Properties of DNA-Linked Gold Nanoparticle Assemblies?Journal of the American Chemical Society, 2003
- What Controls the Optical Properties of DNA-Linked Gold Nanoparticle Assemblies?Journal of the American Chemical Society, 2000
- A DNA-based method for rationally assembling nanoparticles into macroscopic materialsNature, 1996